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Heaton, J.; McCalley, D. V. (2016)
Publisher: Elsevier
Languages: English
Types: Article
Some factors which present difficulties for obtaining good peak shape in hydrophilic interaction chromatography (HILIC) were studied. The effect of injection solvent composition and volume were systematically investigated using a selection of weak and stronger basic compounds on a hybrid bare silica phase. Increasing the mismatch between the injection solvent (range 95 to 0 % ACN, v/v) and the mobile phase (maintained at 95 % ACN v/v) gave increasing deterioration in peak shape. With the 2.1 mm ID columns used, injections in the mobile phase of increasing volume (1-20 μL) gave poorer peak shape, but the magnitude of the effect was considerably smaller than that of solvent mismatch over this range. Some solute structural features such as galloyl (trihydroxy benzene), catechol (benzene diol) and phosphate (in nucleotides) gave serious peak tailing, attributed to interactions with metals in the stationary phase or the chromatographic hardware. These undesirable effects can be moderated by including complexing agents in the mobile phase, by changing the stationary phase chemistry, or by altering the mobile phase pH.
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    • [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [27] [28] [29] [30] [31] [32] [33] [34] [35] Agilent 1260 Infinity Bio-inert Quaternary LC, Agilent Technologies, Wilmington, USA, 2012., n.d. http://www.chem.agilent.com/library/datasheets/public/5990-6129en.pdf.
    • ACQUITY UPLC H-Class Bio System, Waters Corp., Milford, USA, 2013., n.d. http://www.waters.com/webassets/cms/library/docs/720003565en.pdf.
    • H. Sakamaki, T. Uchida, L.W. Lim, T. Takeuchi, Evaluation of column hardware on liquid chromatography-mass spectrometry of phosphorylated compounds, J. Chromatogr. A. 1381 (2015) 125-131
    • M. Andjelkovic, J. van Camp, B. De Meulenaer, G. Depaemelaere, C. Socaciu, M. Verloo, R. Verhe, Iron-chelation properties of phenolic acids bearing catechol and galloyl groups, Food Chem. 98 (2006) 23-31
    • A.M.N. Silva, X. Kong, M.C. Parkin, R. Cammack, R.C. Hider, Iron(III) citrate speciation in aqueous solution, Dalton Trans. (2009) 8616-8625
    • A. Kumar, J.P. Hart, D. V McCalley, Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection., J. Chromatogr. A. 1218 (2011) 3854- 3861
    • R.T. Peaston, C. Weinkove, Measurement of catecholamines and their metabolites, Ann. Clin. Biochem. 41 (2004) 17-38
    • L. Andersson, J. Porath, Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography, Anal. Biochem. 154 (1986) 250-254
    • G. Dobrowolska, G. Muszyńska, J. Porath, Model studies on iron(III) ion affinity chromatography : Interaction of immobilized metal ions with nucleotides, J. Chromatogr. A. 541 (1991) 333-339
    • A. Wakamatsu, K. Morimoto, M. Shimizu, S. Kudoh, A severe peak tailing of phosphate compounds caused by interaction with stainless steel used for liquid chromatography and electrospray mass spectrometry, J. Sep. Sci. 28 (2005) 1823-1830 R. Tuytten, F. Lemière, E. Witters, W. Van Dongen, H. Slegers, R.P. Newton, H. Van Onckelen, E.L. Esmans, Stainless steel electrospray probe: a dead end for phosphorylated organic compounds?, J. Chromatogr. A. 1104 (2006) 209-221
    • Y. Asakawa, N. Tokida, C. Ozawa, M. Ishiba, O. Tagaya, N. Asakawa, Suppression effects of carbonate on the interaction between stainless steel and phosphate groups of phosphate K.T. Myint, T. Uehara, K. Aoshima, Y. Oda, Polar anionic metabolome analysis by nano-LC/MS with a metal chelating agent, Anal. Chem. 81 (2009) 7766-7772
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