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Ali, Alsagher A.O.; Stear, Abigail; Fairlie-Clarke, Karen; Brujeni, Gholamreza Nikbakht; Isa, N. Mahiza Md; Salisi, M. Shahrom; Donskow-Łysoniewska, Katarzyna; Groth, David; Buitkamp, Johannes; Stear, Michael J. (2017)
Publisher: Springer
Journal: Immunogenetics
Languages: English
Types: Article
Subjects: Original Article, Class II region, Texel breed, Polymorphism, Major histocompatibility complex, Sheep
Understanding the structure of the major histocompatibility complex, especially the number and frequency of alleles, loci and haplotypes, is crucial for efficient investigation of the way in which the MHC influences susceptibility to disease. Nematode infection is one of the most important diseases suffered by sheep, and the class II region has been repeatedly associated with differences in susceptibility and resistance to infection. Texel sheep are widely used in many different countries and are relatively resistant to infection. This study determined the number and frequency of MHC class II genes in a small flock of Texel sheep. There were 18 alleles at DRB1, 9 alleles at DQA1, 13 alleles at DQB1, 8 alleles at DQA2 and 16 alleles at DQB2. Several haplotypes had no detectable gene products at DQA1, DQB1 or DQB2, and these were defined as null alleles. Despite the large numbers of alleles, there were only 21 distinct haplotypes in the population. The relatively small number of observed haplotypes will simplify finding disease associations because common haplotypes provide more statistical power but complicate the discrimination of causative mutations from linked marker loci. Electronic supplementary material The online version of this article (doi:10.1007/s00251-016-0962-6) contains supplementary material, which is available to authorized users.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • Atlija M, Gutíerrez-Gil B, Arranz J-J, Semmer J, Stear M, Buitkamp J (2015) Major histocompatibility complex class IIB polymorphism in an ancient Spanish breed. Immunogenetics 67:531-537
    • Ballingall KT, Fardoe K, McKeever DJ (2008) Genomic organisation and allelic diversity within coding and non-coding regions of the OvarDRB1 locus. Immunogenetics 60:95-103
    • Ballingall KT, Rocchi MS, McKeever DJ, Wright F (2010) Trans-species polymorphism and selection in the MHC class II DRA genes of domestic sheep. PLoS One 5:e11402
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    • Bishop SC, Jackson F, Coop RL, Stear MJ (2004) Genetic parameters for resistance to nematode infections in Texel lambs and their utility in breeding programmes. Anim Sci 78:185-194
    • Dukkipati VSR, Blair HT, Garrick DJ, Murray A (2006) BOvar-MHC^- ovine major histocompatibility complex: role in genetic resistance to diseases. N Z Vet J 54:153-160
    • Feichtlbauer-Huber P, Stear MJ, Fries R, Buitkamp J (2000) Referencestrand-mediated conformation analysis of MHC alleles: a new method for high-resolution typing of the Ovar-DQB genes. Immunogenetics 51:65-68
    • Hassan M, Good B, Hanrahan JP, Campion D, Sayers G, Mulcahy G, Sweeney T (2011) The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection. Vet Res 42:46
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    • Hickford JGH, Zhou H, Slow S, Fang Q (2004b) Diversity of the ovine DQA2 gene. J Anim Sci 82:1553-1563
    • Hickford JGH, Zhou H, Fang Q (2007) Haplotype analysis of the DQA genes in sheep: evidence supporting recombination between the loci. J Anim Sci 85:577-582
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    • Lee C, Qin J, Munyard K, Siva Subramaniam N, Wetherall J, Stear M, Groth D (2012) Conserved haplotype blocks within the sheep MHC and low SNP heterozygosity in the class IIa subregion. Anim Genet 43:429-437
    • Nikbakht G, Rezaii H, Stear MJ, Talebi MA, Mahmoudzadeh H (2012) Allelic polymorphism in the second exon of Ovar-DRB1 in fattailed sheep. Vet J 192:547-549
    • Sayers G, Good B, Hanrahan JP, Ryan M, Angles JM, Sweeney T (2005) Major histocompatibility complex DRB1 gene: its role in nematode resistance in Suffolk and Texel sheep breeds. Parasitology 131:403-409
    • Scott PC, Maddox JF, Gogolin-Ewens KJ, Brandon MR (1992) The nucleotide sequence and evolution of ovine MHC class II B genes: DQB and DRB. Immunogenetics 35:217-217
    • Siva Subramaniam N, Morgan E, Wetherall J, Stear M, Groth D (2015) A comprehensive mapping of the structure and gene organisation in the sheep MHC class I region. BMC Genomics 16:1-17
    • Snibson KJ, Maddox JF, Fabb SA, Brandon MR (1998) Allelic variation of ovine MHC class II DQA1 and DQA2 genes. Anim Genet 29:356-362
    • Stear MJ, Innocent GT, Buitkamp J (2005) The evolution and maintenance of polymorphism in the major histocompatibility complex. Vet Immunol Immunopathol 108:53-57
    • Stear M, Belch A, Donskow-Schmelter K, Fitton L, Innocent G, Ishikane C, Mateus A, Murphy L, Rennie K, Smith A (2007) Detection of genes with moderate effects on disease resistance using ovine MHC and resistance to nematodes as an example. Vet Immunol Immunopathol 120:3-9
    • Wegner KM, Reusch TBH, Kalbe M (2003) Multiple parasites are driving major histocompatibility complex polymorphism in the wild. J Evol Biol 16:224-232
  • Discovered through pilot similarity algorithms. Send us your feedback.

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