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Laura Frigotto; Matthew E. Smith; Christopher Brankin; Ashni Sedani; Simon E. Cooper; Nisha Kanwar; Daniel Evans; Stanislava Svobodova; Claudia Baar; Jacob Glanville; Christopher G. Ullman; Anna V. Hine (2015)
Publisher: MDPI AG
Journal: Antibodies
Languages: English
Types: Article
Subjects: CDR, antibody engineering, camelid antibody, RC581-607, saturation mutagenesis, single domain antibody, ProxiMAX randomization, Immunologic diseases. Allergy, Colibra
We have previously described ProxiMAX, a technology that enables the fabrication of precise, combinatorial gene libraries via codon-by-codon saturation mutagenesis. ProxiMAX was originally performed using manual, enzymatic transfer of codons via blunt-end ligation. Here we present Colibra™: an automated, proprietary version of ProxiMAX used specifically for antibody library generation, in which double-codon hexamers are transferred during the saturation cycling process. The reduction in process complexity, resulting library quality and an unprecedented saturation of up to 24 contiguous codons are described. Utility of the method is demonstrated via fabrication of complementarity determining regions (CDR) in antibody fragment libraries and next generation sequencing (NGS) analysis of their quality and diversity.

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