LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Publisher: Cambridge University Press
Languages: English
Types: Article
Subjects: RE, R1
The basic mechanism of reinforcement in tendons addresses the transfer of stress, generated by the deforming proteoglycan (PG)-rich matrix, to the collagen fibrils. Regulating this mechanism involves the interactions of PGs on the fibril with those in the surrounding matrix and between PGs on adjacent fibrils. This understanding is key to establishing new insights on the biomechanics of tendon in various research domains. However, the experimental designs in many studies often involved long sample preparation time. To minimise biological degradation the tendons are usually stored by freezing. Here, we have investigated the effects of commonly used frozen storage temperatures on the mechanical properties of tendons from the tail of a murine model (C57BL6 mouse). Fresh (unfrozen) and thawed samples, frozen at temperatures of −20°C and −80°C, respectively, were stretched to rupture. Freezing at −20°C revealed no effect on the maximum stress (σ), stiffness (E), the corresponding strain (ε) at σ and strain energy densities up to ε (u) and from ε until complete rupture (up). On the other hand, freezing at −80°C led to higher σ, E and u; ε and up were unaffected. The results implicate changes in the long-range order of radially packed collagen molecules in fibrils, resulting in fibril rupture at higher stresses, and changes to the composition of extrafibrillar matrix, resulting in an increase in the interaction energy between fibrils via collagen-bound PGs.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • Bevilacqua A, Zaritzky N and Calvelo A 1979. Histological measurements of ice in frozen beef. Journal of Food Technology 14, 237-251.
    • Chevalier D, Le Bail A and Ghoul M 2000. Freezing and ice crystals formed in a cylindrical food model: part I. Freezing at atmospheric pressure. Journal of Food Engineering 46, 277-285.
    • Derwin KA and Soslowsky LJ 1999. A quantitative investigation of structurefunction relationships in a tendon fascicle model. Journal of Biomechanical Engineering 121, 598-604.
    • Giannini S, Buda R, Di Caprio F, Agati P, Bigi A, De Pasquale V and Ruggeri A 2008. Effects of freezing on the biomechanical and structural properties of human posterior tibial tendons. International Orthopaedics 32, 145-151.
    • Goh KL, Meakin JR, Aspden RM and Hukins DWL 2005. Influence of fibril taper on the function of collagen to reinforce the extra-cellular matrix. Proceedings of the Royal Society of London B272, 1979-1983.
    • Goh KL, Meakin JR, Aspden RM and Hukins DWL 2007. Stress transfer in collagen fibrils reinforcing connective tissues: effects of collagen fibril slenderness and relative stiffness. Journal of Theoretical Biology 245, 305-311.
    • Goh KL, Holmes DF, Lu H-Y, Richardson S, Kadler KE, Purslow PP and Wess TJ 2008. Ageing changes in the tensile properties of tendons: influence of collagen fibril volume fraction. Journal of Biomechanical Engineering 130021011, 1-8.
    • Gondret F, Hernandez P, Remignon H and Combes S 2009. Skeletal muscle adaptations and biomechanical properties of tendons in response to jump exercise in rabbits. Journal of Animal Science 87, 544-553.
    • Hickey DS and Hukins DWL 1979. Effect of methods of preservation on the arrangement of collagen fibrils in connective tissue matrices: an X-ray diffraction study of annulus fibrosus. Connective Tissue Research 6, 223-228.
    • Hukins DWL and Aspden RM 1985. Composition and properties of connective tissues. Trends in Biochemical Sciences 10, 260-264.
    • Laing JH, Cameron GJ and Wess TJ 2003. Molecular organisation of collagen fibrillar structures - a review. Recent Research Developments in Molecular Biology 1, 51-71.
    • Laissue P, L'H oˆte D, Serres C and Vaiman D 2009. Mouse models for identifying genes modulating fertility parameters. Animal 3, 55-71.
    • Lin TW, Cardenas L and Soslowsky LJ 2004. Biomechanics of tendon injury and repair. Journal of Biomechanics 37, 865-877.
    • Miyawaki O, Abe T and Yano T 1992. Freezing and ice structure formed in protein gels. Bioscience, Biotechnology and Biochemistry 56, 953-957.
    • Moussa M, Babile´ R, Fernandez X and Remignon H 2007. Biochemical and biomechanical properties of tendons in two commercial types of chickens.
    • Animal 1, 983-988.
    • Muldrew K and McGann LE 1990. Mechanisms of intracellular ice formation.
    • Biophysical Journal 57, 525-532.
    • Pardo JM, Suess F and Niranjan K 2002. An investigation into the relationship between freezing rate and mean ice crystal size for coffee extracts. Trans IChemE - Food and Bioproducts Processing 80, 176-182.
    • Puxkandl R, Zizak I, Paris O, Keckes J, Tesch W, Bernstorff S, Purslow P and Fratzl P 2002. Viscoelastic properties of collagen: synchrotron radiation investigations and structural model. Philosophical Transactions of the Royal Society of London B357, 191-197.
    • Redaelli A, Vesentini S, Soncini M, Vena P, Mantero S and Montevecchi FM 2003. The possible role of decorin glycosaminoglycans in fibril to fibril force transfer in relative mature tendons - a computational study from molecular to microstructural level. Journal of Biomechanics 36, 1555-1569.
    • Screen HRC, Chhaya VH, Greenwald SE and Bader DL 2006. The influence of swelling and matrix degradation on the microstructural integrity of tendon. Acta Biomaterialia 2, 505-513.
    • Sikoryn TA and Hukins DWL 1988. Failure of the longitundinal ligaments of the spine. Journal of Materials Science Letters 7, 1345-1349.
    • Sun CQ 2009. Thermo-mechanical behavior of low-dimensional systems: the local bond average approach. Progress in Materials Science 54, 179-307.
    • Tiller WA and Rutter JW 1956. The effect of growth conditions upon the solidification of a binary allow. Canadian Journal of Physics 34, 96-121.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article