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Haggag, Yusuf; Abdel-Wahab, Yasser; Ojo, Opeolu; Osman, Mohamed; El-Gizawy, Sanaa; El-Tanani, Mohamed; Faheem, Ahmed; McCarron, Paul (2015)
Publisher: Elsevier
Languages: English
Types: Article

Classified by OpenAIRE into

mesheuropmc: technology, industry, and agriculture
The aim of this study was to design a controlled release vehicle for insulin to preserve its\ud stability and biological activity during fabrication and release. A modified, double emulsion,\ud solvent evaporation, technique using homogenisation force optimised entrapment efficiency of\ud insulin into biodegradable nanoparticles (NP) prepared from poly (dl-lactic-co-glycolic acid)\ud (PLGA) and its PEGylated diblock copolymers. Formulation parameters (type of polymer and\ud its concentration, stabiliser concentration and volume of internal aqueous phase) and\ud physicochemical characteristics (size, zeta potential, encapsulation efficiency, in vitro release\ud profiles and in vitro stability) were investigated. In vivo insulin sensitivity was tested by dietinduced\ud type II diabetic mice. Bioactivity of insulin was studied using Swiss TO mice with\ud streptozotocin-induced type I diabetic profile. Insulin-loaded NP were spherical and negatively\ud charged with an average diameter of 200–400 nm. Insulin encapsulation efficiency increased\ud significantly with increasing ratio of co-polymeric PEG. The internal aqueous phase volume\ud had a significant impact on encapsulation efficiency, initial burst release and NP size.\ud Optimised insulin NP formulated from 10% PEG-PLGA retained insulin integrity in vitro,\ud insulin sensitivity in vivo and induced a sustained hypoglycaemic effect from 3 hours to 6 days\ud in type I diabetic mice.
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