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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Williams, Leanne (2012)
Languages: English
Types: Unknown

Classified by OpenAIRE into

mesheuropmc: endocrine system
The growth of ovarian follicles is well documented in terms of hormonal control, however the fluid dynamics of antral follicle growth is less well understood. Aquaporins (AQP) are transmembrane water channels which facilitate the passive movement of water. In mammals 13 AQPs have been identified in a vast range of tissue types. In terms of ovarian AQPs there is currently a paucity of information. Recent studies in rat, pig and human have revealed the presence of ovarian AQPs, but in doing so have also highlighted a lack of consensus on AQP-type and location. The main aim of this study was to investigate the potential role of AQP in antral follicle growth. The first objective was to identify tissue expression and localisation of AQP proteins in the bovine ovary. This required the characterisation of a panel of polyclonal serum antibodies. Immunohistochemistry (IHC) was then used to identify AQPs and to detect changes in protein expression during follicular growth. Aquaporin 1 was found in most vascular endothelium; it was plentiful in capillaries surrounding antral follicles and increased in abundance as vasculature increased with follicle development. Aquaporin 2 was not found in bovine ovarian tissue and the remaining antibodies were deemed too nonspecific to permit reliable conclusions. The second objective was to investigate, via RT-qPCR, mRNA levels of AQPs in granulosa and theca cells isolated from preantral, through to large preovulatory follicles. Transcripts of AQP 1, -3, -4, -5, -7 and -9 were detected in both the granulosa and theca of antral follicles with expression levels generally higher in theca. The expression of AQP 1, -5, -7 and -9 was initiated in the theca cells of early antral follicles. Finally, swelling assays using bovine and porcine granulosa cells demonstrated the ability of granulosa to swell. This was inhibited by HgCb which is characteristic of AQP function. Porcine granulosa cells incubated with androgen swelled by 27%, this effect was inhibited by hydroxyflutamide. Protein analysis of AQP5 via IHC and Western blotting showed possible up-regulation in porcine follicles. RTqPCR did not reveal AQP5 transcript, the reasons for this currently remain unclear. In conclusion, this study has revealed for the first time the involvement of AQPs in bovine ovarian follicle development, with AQPI, -5, -7 and -9 potentially playing a pivotal role in antrum formation. The AQP system in porcine granulosa cells is androgen sensitive however identification of the AQP/s responsible needs further investigation. The evidence from this investigation suggests a role for AQPs in facilitating follicle growth. The stage-dependent expression of certain AQPs and the androgen sensitive porcine granulosa cells reveals the possibility that AQPs may be modulated by follicle-regulating hormones.
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    • King LS, Kozono D and Agre P 2004 From structure to disease: the evolving tale of aquaporin biology. Nature rev. Mol. Cell Bioi. 5687-698.
    • Knickerbocker JJ, Wiltbank MC and Niswender GD 1988 Mechanisms of luteolysis in domestic livestock. Dom Ani Endocrinol. 5 91 - 107
    • Knight PG and Glister C 200 I Potential local regulatory functions of inhibins, activins and follistatin in the ovary. Reproduction. 121 503-512.
    • Ku DD, Zaleski JK, Liu S and Brock TA 1993 Vascular endothelial growth factor induces EDRF-dependent relaxation in coronary arteries. Am J Physiol. 265 586-592.
    • Kuiper GG, Carlsson B, Grandien K, Enmark E, Haggblad J, Nilsson Sand Gustafsson JA 1997 Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology. 138 863-870.
    • Kuriyama H, Kawamoto S, Ishida N, Ohno I, Mita S, Matsuzawa Y, Matsubara K, Okubo K 1997 Molecular cloning and expression of a novel human aquaporin from adipose tissue with glycerol permeability. Biochem Biophys Res Commun. 241 53-58.
    • Kuriyama H, Shimomura I, Kishida K,H, Furuyama N, Nishizawa H, Maeda N, Matsuda M, Nagaretani H, Kihara S, Nakamura T, Tochino Y, Funahashi T and Matsuzawa Y 2002 Coordinated regulation of fat-specific and liver-specific glycerol channels, aquaporin adipose and aquaporin 9. Diabetes. 51 2915-2921.
    • Laforenza U, Gastaldi G, Grazioli M, Cova E, Tritto S, Faelli A, Calamita G and Ventura U 2005 Expression and localisation of aquaporin-7 in rat gastrointestinal tract. BioI. Cell. 97 605-613.
    • Lammond DR and Drost M 1974 Blood supply to the bovine ovary. J Anim Sci. 38 106- 112.
    • Laurincik J, Kroslak P, Hyttel P, Pivko J and Sirotkin AV 1992 Bovine cumulus expansion and corona-oocyte disconnection during culture in vitro. Reprod Nutr Dev. 32 151-161.
    • Lenton EA, King H, Thomas EJ, Smith SK, McLachlan RI, MacNeil S and Cooke ID 1988 The endocrine environment of the human oocyte. J Reprod Fert. 82827-841.
    • Leonardsson G, Peng XR, Liu K, Nordstrom L, Carmeliet P, Mulligan R, Collen D and Ny T 1995 Ovulation efficiency is reduced in mice that lack plasminogen activator gene function: functional redundancy among physiological plasminogen activators. PNAS USA. 92 12445-12450.
    • Martelli A, Bernabe N, Berardinelli P, Russo V, Rinaldi C, Di Giacinto 0, Mauro A, and Barboni B 2009 Vascular supply as a discriminating factor for pig preantral follicle selection. Reproduction. 13745-58.
    • MM 1995 Functional analysis of mammalian members of the transforming growth factor-beta superfamily. Trends Endocrin Met. 6 120-127.
    • Mauro A 1957 Nature of solvent transfer in osmosis. Science. 126252-3.
    • McArthur ME, Irvin-Rodgers HF, Byers S and Rodgers RJ 2000 Identification and immunolocalisation of decor in, versican, perlecan, nidogen and chondroitin sulphate proteoglycans in bovine small ovarian follicles. Bioi Reprod. 63 913-924 .
    • McConnell NA, Yunus RS, Gross SA, Bost KL, Clements MG and Hughes FM 2002. Water permeability of an ovarian antral follicle is predominantly transcellular and mediated by aquaporins. Endocrinology. 1432905-12.
    • Middleton J, Amerieh L, Gayon R, Julien D, Mansat M, Mansat P, Anraet P, Cantagrel A, Cattan P, Reimund JM, Aguilar L, Amalrie F and Girard JP 2005 A comparative study of endothelial cell markers expressed in chronically inflamed human tissues: MECA-79, Duffy antigen receptor for chemokines, von WiIlebrand factor, CD31, CD34, CD105 and CDI46.J Pathol. 206260-268.
    • Mignotte Band Vayssiere JL 1998 Mitochondria and apoptosis. Eur J Bioehem. 252 1-1 Mihm M, Austin E, Good TEM, Ireland JLH, Knight P, Roehe JF and Ireland JJ 2000 Identification of potential intrafollicular factors involved in selection of dominant follicles in heifers. Bioi. Reprod 63 811-819.
    • Moehren U, Denayer S, Podvinec M, Verrijdt G and Claessens F 2008 Identification of androgen-selective androgen-response elements in the human aquaporin-S and Rad9 genes. Biochem J. 411 679-86.
    • Noda, Y. and S. Sasaki 2006 Regulation of aquaporin-2 trafficking and its binding protein complex. Biochim Biophys Acta. 1758 1117-1125.
    • Norman RJ, Dewailly D, Legro RS and Hickey TE 2007 Polycystic ovarian syndrome. Lancet. 379 685-697.
    • Nuttinck F, Peynot N, HumboIt P, Massip A, Dessy F and Flechon E 2000 Comparative immunohistochemical distribution of connexion 37 and connexion 43 throughout folliculogenesis in the bovine ovary. Mol. Reprod Dev. 5760-66
    • Okahira M, Kubota M, Iguchi K, Usui S and Hirano K 2008 Regulation of aquaporin 3 expression by magnesium ion. Eur J Pharmacol. 588 26-32.
    • Selvey S, Thompson EW, Matthaei K, Lea RA , Irving MG and Griffiths LR 2001 pActin - an unsuitable internal control for RT-PCR. Mol Cell Probe. IS 307-311.
    • Zeidel ML, Ambudkar SV, Smith BL and Agre P 1992 Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein. Biochemistry. 31: 7436-7440.
    • proteins. J Membr BioI. 234 57-73.
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