Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Chen, Weiwei; Kong, Junhua; Qin, Cheng; Yu, Sheng; Tan, Jinjuan; Chen, Yun-ru; Wu, Chaoqun; Wang, Hui; Shi, Yan; Li, Chunyang; Li, Bin; Zhang, Pengcheng; Wang, Ying; Lai, Tongfei; Yu, Zhiming; Zhang, Xian; Shi, Nongnong; Wang, Huizhong; Osman, Toba; Liu, Yule; Manning, Kenneth; Jackson, Stephen D.; Rolin, Dominique; Zhong, Silin; Seymour, G. B.; Gallusci, Philippe; Hong, Yiguo (2015)
Publisher: Nature Publishing Group
Languages: English
Types: Article
Subjects: SB

Classified by OpenAIRE into

mesheuropmc: circulatory and respiratory physiology, cardiovascular system, food and beverages
Naturally-occurring epimutants are rare and have mainly been described in plants. However how these mutants maintain their epigenetic marks and how they are inherited remain unknown. Here we report that CHROMOMETHYLASE3 (SlCMT3) and other methyltransferases are required for maintenance of a spontaneous epimutation and its cognate Colourless non-ripening (Cnr) phenotype in tomato. We screened a series of DNA methylation-related genes that could rescue the hypermethylated Cnr mutant. Silencing of the developmentally-regulated SlCMT3 gene results in increased expression of LeSPL-CNR, the gene encodes the SBP-box transcription factor residing at the Cnr locus and triggers Cnr fruits to ripen normally. Expression of other key ripening-genes was also up-regulated. Targeted and whole-genome bisulfite sequencing showed that the induced ripening of Cnr fruits is associated with reduction of methylation at CHG sites in a 286-bp region of the LeSPL-CNR promoter, and a decrease of DNA methylation in differentially-methylated regions associated with the LeMADS-RIN binding sites. Our results indicate that there is likely a concerted effect of different methyltransferases at the Cnr locus and the plant-specific SlCMT3 is essential for sustaining Cnr epi-allele. Maintenance of DNA methylation dynamics is critical for the somatic stability of Cnr epimutation and for the inheritance of tomato non-ripening phenotype.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • We thank D. C. Baulcombe for providing the original PVX vector. This work was in part supported by a Pandeng Pragramme from Hangzhou Normal University (201108), an Innovative Grant for Science Excellence from Hangzhou City Education Bureau, China and the UK Biotechnology and Biological Sciences Research Council core funding (BBS/E/H/ 00YH0271) to Y.H., and by the General Research Fund 14119814 and the State Key Laboratory of Agrobiotechnology Fund 8300063 to S.Z. G.B.S. was funded from the TomNet project sponsored by the UK Biotechnology and Biological Sciences Research Council. We also thank the National Natural Science Foundation of China (31370180, 31401926, 31200913, 31201490) and the Zhejiang Provincial Natural Science Foundation (LQ13C020004, LQ13C060003, LQ12C02005, LY14C010005) for supports.
  • No related research data.
  • Discovered through pilot similarity algorithms. Send us your feedback.

Share - Bookmark

Cite this article