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Liu, R.; He, X.; Lehane, Stella; Lehane, Mike; Hertz-Fowler, C.; Berriman, M.; Field, L. M.; Zhou, J.-J. (2012)
Publisher: Wiley
Languages: English
Types: Article
Subjects: trypanosomiasis, qx_45, chemosensory protein, qu_500, Original Articles, tsetse fly, qx_505, nagana, gene expression, qu_58.5

Classified by OpenAIRE into

mesheuropmc: fungi, parasitic diseases, animal structures
Chemosensory proteins (CSPs) are a class of soluble proteins present in high concentrations in the sensilla of insect antennae. It has been proposed that they play an important role in insect olfaction by mediating interactions between odorants and odorant receptors. Here we report, for the first time, the presence of five CSP genes in the tsetse fly Glossina morsitans morsitans, a major vector transmitting nagana in livestock. Real-time quantitative reverse transcription PCR showed that three of the CSPs are expressed in antennae. One of them, GmmCSP2, is transcribed at a very high level and could be involved in olfaction. We also determined expression in the antennae of both males and females at different life stages and with different blood feeding regimes. The transcription of GmmCSP2 was lower in male antennae than in females, with a sharp increase in 10-week-old flies, 48 h after a bloodmeal. Thus there is a clear relationship between CSP gene transcription and host searching behaviour. Genome annotation and phylogenetic analyses comparing G. morsitans morsitans CSPs with those of other Diptera showed rapid evolution after speciation of mosquitoes.
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    • Aksoy, S., Berriman, M., Hall, N., Hattori, M., Hide, W. and Lehane, M. (2005) A case for a Glossina genome project. Trends Parasitol 21: 107-111.
    • Angeli, S., Ceron, F., Scaloni, A., Monti, M., Monteforti, G., Minnocci, A. et al. (1999) Purification, structural characterization, cloning and immunocytochemical localization of chemoreception proteins from Schistocerca gregaria. Eur J Biochem 262: 745-754.
    • Arensburger, P., Megy, K., Waterhouse, R.M., Abrudan, J., Amedeo, P., Antelo, B. et al. (2010) Sequencing of Culex quinquefasciatus establishes a platform for mosquito comparative genomics. Science 330: 86-88.
    • Ban, L., Scaloni, A., Brandazza, A., Angeli, S., Zhang, L., Yan, Y. et al. (2003) Chemosensory proteins of Locusta migratoria. Insect Mol Biol 12: 125-134.
    • Briand, L., Swasdipan, N., Nespoulous, C., BĂ©zirard, V., Blon, F., Huet, J.C. et al. (2002) Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier. Eur J Biochem 269: 4586- 4596.
    • Campanacci, V., Mosbah, A., Bornet, O., Wechselberger, R., Jacquin-Joly, E., Cambillau, C. et al. (2001) Chemosensory protein from the moth Mamestra brassicae. Expression and secondary structure from 1H and 15N NMR. Eur J Biochem 268: 4731-4739.
    • Campanacci, V., Lartigue, A., Hallberg, B.M., Jones, T.A., GiudiciOrticoni, M.T., Tegoni, M. et al. (2003) Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding. Proc Natl Acad Sci USA 100: 5069- 5074.
    • Dani, F.R., Michelucci, E., Francese, S., Mastrobuoni, G., Cappellozza, S., La Marca, G. et al. (2011) Odorant-binding proteins and chemosensory proteins in pheromone detection and release in the silkmoth Bombyx mori. Chem Senses 36: 335- 344.
    • ForĂȘt, S., Wanner, K.W. and Maleszka, R. (2007) Chemosensory proteins in the honey bee: insights from the annotated genome, comparative analyses and expressional profiling. Insect Biochem Mol Biol 37: 19-28.
    • Jacquin-Joly, E., Vogt, R.G., Francois, M.C. and Nagnan-Le Meillour, P. (2001) Functional and expression pattern analysis of chemosensory proteins expressed in antennae and pheromonal gland of Mamestra brassicae. Chem Senses 26: 833- 844.
    • Jansen, S., Chmelik, J., Zidek, L., Padrta, P., Novak, P., Zdrahal, Z. et al. (2007) Structure of Bombyx mori chemosensory protein 1 in solution. Arch Insect Biochem Physiol 66: 135-145.
    • Kitabayashi, A.N., Arai, T., Kubo, T. and Natori, S. (1998) Molecular cloning of cDNA for p10, a novel protein that increases in regenerating legs of Periplaneta americana (American cockroach). Insect Biochem Mol Biol 28: 785-790.
    • Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H. et al. (2007) ClustalW and ClustalX version 2. Bioinformatics 23: 2947-2948.
    • Liu, R., Lehane, S., He, X., Lehane, M., Hertz-Fowler, C., Berriman, M. et al. (2010) Characterisations of odorant-binding proteins in the tsetse fly Glossina morsitans morsitans. Cell Mol Life Sci 67: 919-929.
    • Liu, X., Luo, Q., Zhong, G., Rizwan-Ul-Haq, M. and Hu, M. (2010) Molecular characterization and expression pattern of four chemosensory proteins from diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). J Biochem 148: 189- 200.
    • Maleszka, R. and Stange, G. (1997) Molecular cloning, by a novel approach, of a cDNA encoding a putative olfactory protein in the labial palps of the moth Cactoblastis cactorum. Gene 202: 39-43.
    • Mameli, M., Tuccini, A., Mazza, M., Petacchi, R. and Pelosi, P. (1996) Soluble proteins in chemosensory organs of Phasmids. Insect Biochem Mol Biol 26: 875-882.
    • McKenna, M.P., Hekmat-Scafe, D.S., Gaines, P. and Carlson, J.R. (1994) Putative D. melanogaster pheromone-binding proteins expressed in a subregion of the olfactory system. J Biol Chem 269: 16340-16347.
    • Marchler-Bauer, A., Lu, S., Anderson, J.B., Chitsaz, F., Derbyshire, M.K., DeWeese-Scott, C. et al. (2011) CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 39(D): 225-229.
    • Moloo, S.K. (1971) An artificial feeding technique for Glossina. Parasitology 63: 507-551.
    • Mosbah, A., Campanacci, V., Lartigue, A., Tegoni, M., Cambillau, C. and Darbon, H. (2003) Solution structure of a chemosensory protein from the moth Mamestra brassicae. Biochem J 369: 39-44.
    • Nagnan-Le Meillour, P., Cain, A.H., Jacquin-Joly, E., Francois, M.C., Ramachandran, S., Maida, R. et al. (2000) Chemosensory proteins from the proboscis of Mamestra brassicae. Chem Senses 25: 541-553.
    • Pelletier, J. and Leal, W.S. (2011) Characterization of olfactory genes in the antennae of the Southern house mosquito, Culex quinquefasciatus. J Insect Physiol 57: 915-929.
    • Pelosi, P., Zhou, J.J., Ban, L.P. and Calvello, M. (2006) Soluble proteins in insect chemical communication. Cell Mol Life Sci 63: 1658-1676.
    • Picimbon, J.-F.J., Dietrich, K., Krieger, J. and Breer, H. (2001) Identity and expression pattern of chemosensory proteins in Heliothis virescens (Lepidoptera, Noctuidae). Insect Biochem Mol Biol 31: 1173-1181.
    • Pikielny, C.W., Hasan, G., Rouyer, F. and Rosbash, M. (1994) Members of a family of Drosophila putative odorant-binding proteins are expressed in different subsets of olfactory hairs. Neuron 12: 35-49.
    • Sabatier, L., Jouanguy, E., Dostert, C., Zachary, D., Dimarcq, J.L., Bulet, P. et al. (2003) Pherokine-2 and -3: two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections. Eur J Biochem 270: 3398-3407.
    • Tomaselli, S., Crescenzi, O., Sanfelice, D., Wechselberger, R., Angeli, S., Scaloni, A. et al. (2006) Solution structure of a chemosensory protein from the desert locust Schistocerca gregaria. Biochemistry 45: 10606-10613.
    • Whitfield, C.W., Band, M.R., Bonaldo, M.F., Kumar, C.G., Liu, L., Pardinas, J. et al. (2002) Annotated expressed sequence tags and cDNA microarrays for studies of brain and behaviours in the honey bee. Genome Res 12: 555-566.
    • Xu, Y.L., He, P., Zhang, L., Fang, S.Q., Dong, S.L., Zhang, Y.J. et al. (2009) Large-scale identification of odorant-binding proteins and chemosensory proteins from expressed sequence tags in insects. BMC Genomics 25: 10-632.
    • Zhou, J.-J., Huang, W., Zhang, G.A., Pickett, J.A. and Field, L.M. (2004) 'Plus-C'' odorant-binding protein genes in two Drosophila species and the malaria mosquito Anopheles gambiae. Gene 327: 117-129.
    • Zhou, J.-J., Kan, Y., Antoniw, J., Pickett, J.A. and Field, L.M. (2006) Genome and EST Analyses and Expression of a Gene Family with Putative Functions in Insect Chemoreception. Chem Senses 31: 453-465.
    • Zhou, J.-J., He, X.L., Pickett, J.A. and Field, L.M. (2008) Identification of odorant-binding proteins of the yellow fever mosquito Aedes aegypti, genome annotation and comparative analyses. Insect Mol Biol 17: 147-163.
    • Zhou, J.-J., Robertson, G., He, X.L., Dufour, S., Hooper, A.M., Pickett, J.A. et al. (2009) Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorantbinding protein discriminates between sex pheromone components. J Mol Biol 389: 529-545.
    • Additional Supporting Information may be found in the online version of this article under the DOI reference: 10.1111/j.1365-2583.2011.01114.x
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