Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Whitaker, Richard George
Languages: English
Types: Doctoral thesis
Subjects: QD, QP
The work presented in this thesis is of two types. Firstly methods for the\ud electrochemical immobilisation of redox enzymes in organic polymers are described.\ud The electrochemical monitoring of the immobilised enzyme reaction by detection of\ud one of the enzyme's products is discussed, and the results obtained for such a system\ud under a variety of experimental conditions are presented.\ud A good understanding of the way in which such a system operates' was\ud obtained by using a specially developed kinetic model., This model is explained\ud fully in the theory chapter of this thesis.\ud A variety of organic polymers were used in the electrochemical\ud immobilisation process, with varying degrees of success. The flexibility of this\ud approach is demonstrated by the use of a variety of immobilisation matrices and also\ud by the development of bienzyme and bilayer devices.\ud The final experimental chapter presents work on the covalent modification of\ud redox enzymes with a variety of, redox centres based. on ferrocene. Although\ud attempts to electrochemically immobilise a modified enzyme were not successful,\ud some interesting information about the kinetic behaviour and stability of a series Of\ud modified enzymes was obtained.\ud An indication of possible work forming an extension to this thesis is given in\ud the final part of this thesis. The electrochemical immobilisation techniques and the\ud procedure for covalently modifying, enzymes using electroactive, groups are\ud relatively recent ideas. Much work remains to be done before a better understanding\ud of these systems is gained.
  • No references.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article