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De Andrade, Cristiano J.; Barros, Francisco F. C.; De Andrade, Lidiane M.; Roco, Silvana A.; Sforca, Mauricio L.; Pastore, Glaucia M.; Jauregi, Paula (2016)
Publisher: Wiley
Languages: English
Types: Article
Subjects:
Identifiers:doi:10.1002/jctb.4928
BACKGROUND: Bacillus subtilis synthesizes surfactin, a powerful surface-active agent. It has interesting potential applications. However, due to its high cost of production, commercial use is impracticable. The downstream processing represents ≈60% of production costs and the culture medium ≈30%. Many reports focused, separately, on production of surfactin using by-products (reduced cost) or the purification using synthetic medium. Therefore, the aim of this work was to evaluate, for the first time, the impact of using a by-product as fermentation medium on the downstream processing based on membrane filtration.\ud RESULTS: Membranes of PES-100-kDa efficiently retained surfactin micelles - the first step of ultrafiltration, whereas, the second step required membranes of 50-kDa to separate surfactin monomers from proteins. Ultrafiltration of crude biosurfactant was associated with fouling and/or concentration polarization resulting in lower purity than when synthetic medium was used. Further improvement in purity was achieved by partial removal of proteins prior to ultrafiltration by precipitation and extraction. The RMN and MALDI-TOFMS analyses identified 11 potential surfactin homologous composed by two amino acid sequences.\ud CONCLUSION: Production of surfactin using cassava wastewater as a low-cost culture medium and its purification by the 2-step ultrafiltration process is feasible, nevertheless, the higher protein content of this medium as compared to the synthetic one leads to a lower purity product; further increase in purity can be achieved by applying additional purification steps prior to ultrafiltration with the subsequent increased in process cost.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Barros FFC, Ponesi AN and Pastore GM, Production of biosurfactant by Bacillus subtilis LB5a on a pilot scale using cassava wastewater as substrate. J Ind Microbiol Biotechnol 35:1071-1078 (2008). DOI: 10.1007/s10295-008-0385-y
    • 2. Makkar RS, Cameotra SS and Banat IM, Advances in utilization of renewable substrates for biosurfactant production. AMB Express 1:1-19 (2011). DOI: 10.1186/2191-0855-1-5
    • 3. Pacwa-Płociniczak M, Płaza GA, Piotrowska-Seget Z and Cameotra SS, Environmental applications of biosurfactants: Recent Advances. Int J Mol Sci 12:633-654. (2011). DOI: 10.3390/ijms12010633
    • 4. Chen HL, Chen YS and Juang, RS, Recovery of surfactin from fermentation broths by a hybrid salting-out and membrane filtration process. Sep Purif Technol 59:244-252. (2008). DOI: 10.1016/j.seppur.2007.06.010
    • 5. Saharan BS, Sahu RK and Sharma D, A review on biosurfactants: fermentation, current developments and perspectives. Genet Eng Biotechnol J 2011:1-14. (2012).
    • 6. Jauregi P, Coutte F, Catiau L, Lecouturier D and Jacques P, Micelle size characterization of lipopeptides produced by B. subtilis and their recovery by the two-step ultrafiltration process Sep Purif Technol 104:175-182. (2013). DOI:10.1016/j.seppur.2012.11.017
    • 7. Chen HL, Chen YS and Juang, RS, Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes. J Membr Sci 299:114-121. (2007). DOI: 10.1016/j.memsci.2007.04.031
    • 8. Chen HL, Chen YS and Juang RS, Flux decline and membrane cleaning in cross-flow ultrafiltration of treated fermentation broths for surfactin recovery. Sep Purif Technol 62:47- 55. (2008). DOI:10.1016/j.seppur.2007.12.015
    • 9. Isa MHM, Coraglia DE, Frazier RA and Jauregi P, Recovery and purification of surfactin from fermentation broth by a two step ultrafiltration process. J Membr Sci 296:51-57. (2007). DOI:10.1016/j.memsci.2007.03.023
    • 10. Isa MHM, Frazier RA and Jauregi P. A further study of the recovery and purification of surfactin from fermentation broth by membrane filtration. Sep Purif Technol 64:176-182. (2008). DOI:10.1016/j.seppur.2008.09.008
    • 11. Lin S-C and Jiang H-J, Recovery and purification of the lipopetide biosurfactant of Bacillus subtilis by ultrafiltration. Biotechnol Tech 11:413-416. (1997). DOI: 10.1023/A:1018468723132
    • 12. Fahim S, Dimitrov K, Gancel F, Vauchel P, Jacques P and Nikov I, Impact of energy supply and oxygen transfer on selective lipopeptide production by Bacillus subtilis BBG21. Bioresour Technol 126:1-6. (2012). DOI: 10.1016/j.biortech.2012.09.019
    • 14. Ayed HB, Hmidet N, Béchet M, Chollet M, Chataigné G, Leclère V and Jacques P, Identification and biochemical characteristics of lipopeptides from Bacillus mojavensis A21. Process Biochem 49:1699-1707. (2014). DOI: 10.1016/j.procbio.2014.07.001
    • 15. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J and Bax A, NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277- 293. (1995). DOI: 10.1007/BF00197809
    • 16. Johnson BA and Blevins RA, NMR View: A computer program for the visualization and analysis of NMR data. J Biomol NMR 4:603-614. (1994). DOI: 10.1007/BF00404272.
    • 17. Ghribi D and Ellouze-Chaabouni S, Enhancement of Bacillus subtilis lipopeptide biosurfactants production through optimization of medium composition and adequate control of aeration. Biotechnol Res Int 2011:1-6. (2011). DOI: 10.4061/2011/653654
    • 18. Zimmermann HF, Anderlei T, Buchs J and Binder M, Oxygen limitation is a pitfall during screening for industrial strains. Appl Microbiol Biotechnol 72:1157-1160. (2006).
    • 19. Abdel-Wawgoud AM, Aboulwafa MM and Hassouna NAH, Characterization of surfactin produced by Bacillus subtilis isolate BS5. Appl Biochem Biotechnol 150:289-303. (2008). DOI: 10.1007/s12010-008-8153-z
    • 20. Knoblich A, Matsumoto M, Ishiguro R, Murata K, Fujiyoshi Y, Ishigami Y and Osman M, Electron cryo-microscopic studies on micellar shape and size of surfactin, an anionic lipopeptide. Colloid Surfaces B 5:43-48. (1995). DOI:10.1016/0927-7765(95)01207-Y
    • 21. Davis DA, Lynch HC and Varley J, The production of surfactin in batch culture by Bacillus subtilis ATCC 21332 is strongly influenced by the conditions of nitrogen metabolism. Enzyme Microb Tech 25:322-329. (1999). DOI:10.1016/S0141-0229(99)00048-4
    • 22. Grangemard I, Peypoux F, Wallach J, Das BC, Labbe H, Caille A, Genest M, MargerDana R, Ptak M and Bonmatin J-M, Lipopetides with improved properties: Structure by NMR, purification by HPLC and structure-activity relationship of new isoleucyl-rich surfactin. J Pept Sci 2:1-10. (1997). DOI: 10.1002/(SICI)1099-1387(199703)3:2<145::AIDPSC96>3.0.CO;2-Y
    • 23. Peypoux F, Bonmatin J-M, Labbe H, Grangemard I, Das BC, Ptak M, Wallach J and spectroscopies. Eur J Biochem 224:89-96. (1994). DOI: 10.1111/j.1432-1033.1994.tb19998.x
    • 24. Korenblum E, Araujo LV, Guimarães CR, Souza LM, Sassaki G, Abreu F, Nitscke M,
    • 25. Maróstica Jr, MR and Pastore, GM, Production of R-(+)-α-terpineol by the
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