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Noble, Ralph; Blackburn, J.; Thorp, G.; Dobrovin-Pennington, Andreja; Pietravalle, S.; Kerins, G.; Allnutt, T. R.; Henry, C. M. (2011)
Publisher: Wiley-Blackwell Publishing Ltd.
Languages: English
Types: Article
Subjects: QK

Classified by OpenAIRE into

mesheuropmc: fungi, complex mixtures
Temperature and exposure time effects on Phytophthora kernoviae and Phytophthora ramorum viability were examined in flasks of compost and in a large-scale composting system containing plant waste. Cellophane, rhododendron leaf and peat-based inoculum of P. kernoviae and P. ramorum isolates were used in flasks; naturally infected leaves were inserted into a large-scale system. Exposures of 5 and 10 days respectively at a mean temperature of 35 degrees C in flask and large-scale composts reduced P. kernoviae and P. ramorum inocula to below detection limits using semi-selective culturing. Although P. ramorum was undetectable after a 1-day exposure of inoculum to compost at 40 degrees C in flasks, it survived on leaves exposed to a mean temperature of 40.9 degrees C for 5 days in a large-scale composting system. No survival of P. ramorum was detected after exposure of infected leaves for 5 days to a mean temperature of >= 41.9 degrees C (32.8 degrees C for P. kernoviae) or for 10 days at >= 31.8 degrees C (25.9 degrees C for Phytophthora pseudosyringae on infected bilberry stems) in large-scale systems. Fitted survival probabilities of P. ramorum on infected leaves exposed in a large-scale system for 5 days at 45 degrees C or for 10 days at 35 degrees C were <3%, for an average initial infection level of leaves of 59.2%. RNA quantification to measure viability was shown to be unreliable in environments that favour RNA preservation: high levels of ITS1 RNA were recovered from P. kernoviae- and P. ramorum-infected leaves exposed to composting plant wastes at >53 degrees C, when all culture results were negative.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • Beales PA, Giltrap PM, Payne A, Ingram N, 2009. A new threat to UK heathland from Phytophthora kernoviae on Vaccinium myrtillus in the wild. Plant Pathology 58, 393.
    • Beales PA, Giltrap PM, Webb KM, Ozolina A, 2010. A further threat to UK heathland bilberry (Vaccinium myrtillus) by Phytophthora pseudosyringae. Plant Pathology 59, 406.
    • Carlson T, Christian N, Bonner JJ, 1999. A role for RNA metabolism in inducing the heat shock response. Gene Expression 7, 283-291.
    • Chimento A, Cacciola SO, Garbelotto M, 2008. Detection of mRNA by reverse transcription PCR as an indicator of viability in Phytophthora ramorum. In: Frankl SJ, Kliejunas JT, Palmieri KM, eds. Proceedings of the Third Sudden Oak Death Science Symposium, 2007. Albany, CA: Gen. Tech. Rep. PSW-GTR-214, U.S. Department of Agriculture, Forest Service, 85-92.
    • de Leon P, Mellado RP, 1997. Ribosomal RNA synthesis in Streptomyces lividans under heat shock conditions. Gene 194, 125-132.
    • Downer AJ, Crohn D, Faber B, Daugovish O, Becker JO, Menge JA, Mochizuki MJ, 2009. Survival of plant pathogens in static piles of ground green waste. Phytopathology 98, 547-554.
    • Fichtner EJ, 2009. Survival, dispersal, and potential soil-mediated suppression of Phytophthora ramorum in a California redwood-tanoak forest. Phytopathology 99, 608-619.
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  • Discovered through pilot similarity algorithms. Send us your feedback.

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