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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Ng, Keng Wooi; Lau, Wing Man; Williams, Adrian C. (2015)
Publisher: Springer US
Languages: English
Types: Article
Immunodiagnostic microneedles provide a novel way to extract protein biomarkers from the skin in a minimally invasive manner for analysis in vitro. The technology could overcome challenges in biomarker analysis specifically in solid tissue, which currently often involves invasive biopsies. This study describes the development of a multiplex immunodiagnostic device incorporating mechanisms to detect multiple antigens simultaneously, as well as internal assay controls for result validation. A novel detection method is also proposed. It enables signal detection specifically at microneedle tips and therefore may aid the construction of depth profiles of skin biomarkers. The detection method can be coupled with computerised densitometry for signal quantitation. The antigen specificity, sensitivity and functional stability of the device were assessed against a number of model biomarkers. Detection and analysis of endogenous antigens (interleukins 1α and 6) from the skin using the device was demonstrated. The results were verified using conventional enzyme-linked immunosorbent assays. The detection limit of the microneedle device, at ≤10 pg/mL, was at least comparable to conventional plate-based solid-phase enzyme immunoassays.
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    • [1] Gremel G, Grannas K, Sutton LA, Pontén F, Zieba A (2013) In situ Protein Detection for Companion Diagnostics. Front Oncol 3:271.
    • [5] Skehel JM (2004) Preparation of extracts from animal tissues. In: Cutler P (ed) Protein Purification Protocols, 2nd ed. Humana Press, Totowa, NJ, pp 15-20
    • [6] Kim Y-C, Park J-H, Prausnitz MR (2012) Microneedles for drug and vaccine delivery. Adv Drug Deliv Rev 64:1547-1568.
    • [7] Tuan-Mahmood T-M, McCrudden MTC, Torrisi BM, McAlister E, Garland MJ, Singh TRR, Donnelly RF (2013) Microneedles for intradermal and transdermal drug delivery. Eur J Pharm Sci 50:623-637.
    • [8] Gill HS, Denson DD, Burris BA, Prausnitz MR (2008) Effect of microneedle design on pain in human volunteers. Clin J Pain 24:585-594.
    • [9] Haq MI, Smith E, John DN, Kalavala M, Edwards C, Anstey A, Morrissey A, Birchall JC (2009) Clinical administration of microneedles: skin puncture, pain and sensation. Biomed Microdevices 11:35-47.
    • [10] Bhargav A, Muller DA, Kendall MAF, Corrie SR (2012) Surface modifications of microprojection arrays for improved biomarker capture in the skin of live mice. ACS Appl Mater Interfaces 4:2483-2489.
    • [11] Coffey JW, Corrie SR, Kendall MAF (2013) Early circulating biomarker detection using a wearable microprojection array skin patch. Biomaterials 34:9572-9583.
    • [12] Lee KT, Muller DA, Coffey JW, Robinson KJ, McCarthy JS, Kendall MAF, Corrie SR (2014) Capture of the Circulating Plasmodium falciparum Biomarker HRP2 in a Multiplexed Format, via a Wearable Skin Patch. Anal Chem 86:10474-10483.
    • [13] Yeow B, Coffey JW, Muller DA, Grøndahl L, Kendall MAF, Corrie SR (2013) Surface modification and characterization of polycarbonate microdevices for capture of circulating biomarkers, both in vitro and in vivo. Anal Chem 85:10196-10204.
    • [14] Muller DA, Corrie SR, Coffey J, Young PR, Kendall MA (2012) Surface modified microprojection arrays for the selective extraction of the dengue virus NS1 protein as a marker for disease. Anal Chem 84:3262-3268.
    • [15] Corrie SR, Fernando GJP, Crichton ML, Brunck MEG, Anderson CD, Kendall MAF (2010) Surface-modified microprojection arrays for intradermal biomarker capture, with low non-specific protein binding. Lab Chip 10:2655.
    • [16] Strambini LM, Longo A, Diligenti A, Barillaro G (2012) A minimally invasive microchip for transdermal injection/sampling applications. Lab Chip 12:3370.
    • [17] Li CG, Lee CY, Lee K, Jung H (2013) An optimized hollow microneedle for minimally invasive blood extraction. Biomed Microdevices 15:17-25.
    • [18] Strambini LM, Longo A, Scarano S, Prescimone T, Palchetti I, Minunni M, Giannessi D, Barillaro G (2015) Self-powered microneedle-based biosensors for pain-free high-accuracy measurement of glycaemia in interstitial fluid. Biosens Bioelectron 66:162-168.
    • [19] Romanyuk AV, Zvezdin VN, Samant P, Grenader MI, Zemlyanova M, Prausnitz MR (2014) Collection of Analytes from Microneedle Patches. Anal Chem 86:10520-10523.
    • [20] Donnelly RF, Mooney K, Caffarel-Salvador E, Torrisi BM, Eltayib E, McElnay JC (2014) Microneedle-Mediated Minimally Invasive Patient Monitoring. Ther Drug Monit 36:10-17.
    • [21] McAllister DV, Wang PM, Davis SP, Park J-H, Canatella PJ, Allen MG, Prausnitz MR (2003) Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: fabrication methods and transport studies. Proc Natl Acad Sci U S A 100:13755-13760.
    • [22] Zhu Y, Gao C, Liu X, Shen J (2002) Surface modification of polycaprolactone membrane via aminolysis and biomacromolecule immobilization for promoting cytocompatibility of human endothelial cells. Biomacromolecules 3:1312-1319.
    • [23] Cai N, Gong Y, Chian KS, Chan V, Liao K (2008) Adhesion dynamics of porcine esophageal fibroblasts on extracellular matrix protein-functionalized poly(lactic acid). Biomed Mater 3:015014.
    • [24] Ansari AA, Hattikudur NS, Joshi SR, Medeira MA (1985) ELISA solid phase: Stability and binding characteristics. J Immunol Methods 84:117-124.
    • [25] Schramm W, Yang T, Midgley AR (1987) Monoclonal antibodies used in solid-phase and liquid-phase assays, as exemplified by progesterone assay. Clin Chem 33:1331-1337.
    • [26] Lakowicz JR (2006) Principles of Fluorescence Spectroscopy. Springer US, Boston, MA, pp 277-330.
    • [27] Shaner NC, Steinbach PA, Tsien RY (2005) A guide to choosing fluorescent proteins. Nat Methods 2:905-909.
    • [28] Bal SM, Caussin J, Pavel S, Bouwstra JA (2008) In vivo assessment of safety of microneedle arrays in human skin. Eur J Pharm Sci 35:193-202.
    • [29] Del Pilar Martin M, Weldon WC, Zarnitsyn VG, Koutsonanos DG, Akbari H, Skountzou I, Jacob J, Prausnitz MR, Compans RW (2012) Local Response to Microneedle-Based Influenza Immunization in the Skin. mBio 3:e00012-12.
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