LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Fernandez Moreira, Vanesa
Languages: English
Types: Doctoral thesis
Subjects: QD
This thesis describes the development of a new variety of lumophores, in particular luminescent rhenium(I) complexes of the type Re(bisim)(CO)3L n+, where bisim represents a bisimine derivative, e.g. bipyridine, and Lisa pyridine derivative (n = 1) or a chloride (n = 0), suitable for specific cell imaging and whose modified photophysical properties overcome some of the problems associated with traditional imaging agents (small Stokes shift, short lifetimes, etc). Specifically the successful synthesis and photophysical studies of a chemical model to validate the concept of a membrane permeable cell-imaging device, fac-Re0)ipyXCO)3(PyCH2COO(CH2)3OTBD) +, are presented. Further investigations towards and improved cell imaging agent, considering different issues like water solubility, lipophilicity and cell encapsulation rate, are then undertaken. Consequently, a new range of water soluble rhenium complexes, ykc- Re(bisimXCO)3(L) n" where bisim is a water soluble bathophenanthroline and L is a pyridine derivative (n = 2) or a chloride (n = 1), as well as a variety of novel yac-Re(bisimXCO)3Cl complexes, where bisim is a terpyridine derivative bearing a self destruction device which can be triggered by nitroreductase, are also presented. Additionally, the synthesis and structural description of a trinuclear rhenium complex is reported, along with a discussion of its use in inclusion experiments and attempts at analogue formation. The design and synthesis of a thiol selective luminescent rhenium complex, fac-Re(bipyXCO)3(PyCH2Cl) +, is described, along with the demonstration of its thiol selectivity both in vitro and in vivo as demonstrated by specific mitochondrial localization. Finally, studies of membrane permeability in liposome models, cellular uptake and localization in different cells such as Spironucleous vortens, yeast cells and human breast cancer cells of a variety of rhenium complexes above cited are also reported. These results combine to demonstrate the promising future of this new class of imaging probes as specific cell imaging agents and therefore, this work represents the initial step in the growing area of the development of transition metal based imaging agents.

Share - Bookmark

Cite this article