LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Murton, Andrew J.; Billeter, R.; Stephens, Francis B.; Des Etages, S.G.; Graber, F.; Hill, R.J.; Marimuthu, Kanagaraj; Greenhaff, Paul L. (2014)
Publisher: American Physiological Society
Languages: English
Types: Article
Subjects:
We sought to ascertain the time course of transcriptional events that occur in human skeletal muscle at the outset of resistance exercise (RE) training in RE naive individuals and determine whether the magnitude of response was associated with exercise-induced muscle damage. Sixteen RE naive men were recruited; eight underwent two sessions of 5 × 30 maximum isokinetic knee extensions (180°/s) separated by 48 h. Muscle biopsies of the vastus lateralis, obtained from different sites, were taken at baseline and 24 h after each exercise bout. Eight individuals acted as nonexercise controls with biopsies obtained at the same time intervals. Transcriptional changes were assessed by microarray and protein levels of heat shock protein (HSP) 27 and αB-crystallin in muscle cross sections by immunohistochemistry as a proxy measure of muscle damage. In control subjects, no probe sets were significantly altered (false discovery rate < 0.05), and HSP27 and αB-crystallin protein remained unchanged throughout the study. In exercised subjects, significant intersubject variability following the initial RE bout was observed in the muscle transcriptome, with greatest changes occurring in subjects with elevated HSP27 and αB-crystallin protein. Following the second bout, the transcriptome response was more consistent, revealing a cohort of probe sets associated with immune activation, the suppression of oxidative metabolism, and ubiquitination, as differentially regulated. The results reveal that the initial transcriptional response to RE is variable in RE naive volunteers, potentially associated with muscle damage and unlikely to reflect longer term adaptations to RE training. These results highlight the importance of considering multiple time points when determining the transcriptional response to RE and associated physiological adaptation.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 22. Smyth GK. Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3: Article3, 2004.
    • 23. Staron RS, Hikida RS, Murray TF, Nelson MM, Johnson P, and Hagerman F. Assessment of skeletal muscle damage in successive biopsies from strength-trained and untrained men and women. European journal of applied physiology and occupational physiology 65: 258-264, 1992.
    • 24. Stepto NK, Coffey VG, Carey AL, Ponnampalam AP, Canny BJ, Powell D, and Hawley JA. Global gene expression in skeletal muscle from well-trained strength and endurance athletes. Med Sci Sports Exerc 41: 546-565, 2009.
    • 25. Stupka N, Tarnopolsky MA, Yardley NJ, and Phillips SM. Cellular adaptation to repeated eccentric exercise-induced muscle damage. J Appl Physiol 91: 1669-1678, 2001.
    • 26. Timmons JA. Variability in training-induced skeletal muscle adaptation. J Appl Physiol 110: 846-853, 2011.
    • 27. Trappe TA, White F, Lambert CP, Cesar D, Hellerstein M, and Evans WJ. Effect of ibuprofen and acetaminophen on postexercise muscle protein synthesis. Am J Physiol Endocrinol Metab 282: E551-556, 2002.
    • 28. Vierck J, O'Reilly B, Hossner K, Antonio J, Byrne K, Bucci L, and Dodson M. Satellite cell regulation following myotrauma caused by resistance exercise. Cell biology international 24: 263-272, 2000.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article