Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Green, C. M. (2006)
Publisher: Elsevier
Languages: English
Types: Article
Subjects: QD, QH301, R1
To prevent duplication or loss of genomic regions during DNA replication, it is essential that the entire genome is copied precisely once every S phase. Cells achieve this by mutually exclusive regulation of origin firing and licensing. A crucial protein that is involved in origin licensing is chromatin licensing and DNA replication factor 1 (CDT1) and, therefore, activity of this protein must be strictly controlled. Four recent articles have demonstrated that proliferating cell nuclear antigen (PCNA), an essential sliding clamp used in replication and DNA repair, has a crucial role in this process by mediating the proteasomal degradation of CDT1.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 1. Blow, J.J. and A. Dutta, Preventing re-replication of chromosomal DNA. Nat Rev Mol Cell Biol, 2005. 6(6): p. 476-486.
    • 2. Diffley, J.F., Regulation of early events in chromosome replication. Curr Biol, 2004. 14(18): p. R778-786.
    • 3. Saxena, S. and A. Dutta, Geminin-Cdt1 balance is critical for genetic stability. Mutat Res, 2005. 569(1-2): p. 111-121.
    • 4. Hu, J., et al., Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage. Nat Cell Biol, 2004. 6(10): p. 1003-1009.
    • 5. Kondo, T., et al., Rapid degradation of Cdt1 upon UV-induced DNA damage is mediated by SCFSkp2 complex. J Biol Chem, 2004. 279(26): p. 27315- 27319.
    • 6. Li, X., et al., The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation. J Biol Chem, 2003. 278(33): p. 30854-30858.
    • 7. Zhong, W., et al., CUL-4 ubiquitin ligase maintains genome stability by restraining DNA-replication licensing. Nature, 2003. 423(6942): p. 885-889.
    • 8. Arias, E.E. and J.C. Walter, PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication. Nat Cell Biol, 2006. 8(1): p. 84-90.
    • 9. Hu, J. and Y. Xiong, An evolutionarily conserved function of proliferating cell nuclear antigen for Cdt1 degradation by the Cul4-Ddb1 ubiquitin ligase in response to DNA damage. J Biol Chem, 2006. 281(7): p. 3753-3756.
    • 10. Nishitani, H., et al., Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. Embo J, 2006. 25(5): p. 1126-1136.
    • 11. Senga, T., et al., PCNA is a cofactor for Cdt1 degradation by CUL4/DDB1- mediated N-terminal ubiquitination. J Biol Chem, 2006. 281(10): p. 6246- 6252.
    • 12. Maga, G. and U. Hubscher, Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J Cell Sci, 2003. 116(Pt 15): p. 3051-3060.
    • 13. Warbrick, E., The puzzle of PCNA's many partners. Bioessays, 2000. 22(11): p. 997-1006.
    • 14. Arias, E.E. and J.C. Walter, Replication-dependent destruction of Cdt1 limits DNA replication to a single round per cell cycle in Xenopus egg extracts. Genes Dev, 2005. 19(1): p. 114-126.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article