Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Sharma, A; Rees, D; Roberts, S; Kuiper, NJ (2017)
Publisher: Elsevier
Languages: English
Types: Article
Subjects: R1

Classified by OpenAIRE into

mesheuropmc: education
Background\ud Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects in thousands of patients worldwide with good clinical effectiveness 10–20 years after implantation. Information concerning the quality of the repair cartilage is still limited because biopsies are small and rare. Glycosaminoglycan structure influences physiological function and is likely to be important in the long term stability of the repair tissue. The aim of this study was to assess glycosaminoglycans in ACI tissue over a two year period.\ud \ud Methods\ud Biopsies were taken from one patient (25 years old) at 12 months and 20 months post-ACI-treatment and from three normal cadavers (21, 22 and 25 years old). Fluorophore-assisted carbohydrate electrophoresis (FACE) was used to quantitatively assess the individual glycosaminoglycans.\ud \ud Results\ud At 12 months the ACI biopsy had 40% less hyaluronan than the age-matched cadaveric biopsies but by 20 months the ACI biopsy had the same amount of hyaluronan as the controls. Both the 12 and 20 month ACI biopsies had less chondroitin sulphate disaccharides and shorter chondroitin sulphate chains than the age-matched cadaveric biopsies. However, chondroitin sulphate chain length doubled as the ACI repair tissue matured at 12 months (3913 Da ± 464) and 20 months (6923 Da ± 711) and there was less keratan sulphate as compared to the controls.\ud \ud Conclusions\ud Although the glycosaminoglycan composition of the repair tissue is not identical to mature articular cartilage its quality continues to improve with time.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • 2. Peterson L, Minas T, Brittberg M, Nilsson A, Sjogren-Jansson E, Lindahl A. Two- to 9-year outcome after autologous chondrocyte transplantation of the knee. Clin Orthop Relat Res. 2000;(374):212-234.
    • 3. Peterson L, Vasiliadis HS, Brittberg M, Lindahl A. Autologous chondrocyte implantation: a long-term follow-up. Am J Sports Med. 2010;38(6):1117-24.
    • 4. Ashvin K. Dewan, Matthew A. Gibson, Jennifer H. Elisseeff, Michael E. Trice. Evolution of Autologous Chondrocyte Repair and Comparison to Other Cartilage Repair Techniques. Biomed Res Int. 2014; 2014: 272481
    • 5. Gerjo J V M Van Osch, Mats Brittberg, James E Dennis, Yvonne M Bastiaansen-Jenniskens, Reinhold G Erben, Yrjö T Konttinen, Frank P Luyten. Cartilage repair: past and future - lessons for regenerative medicine. J Cell Mol Med. 2009 May; 13(5): 792-810.
    • 6. Clinical Outcome of Autologous Chondrocyte Implantation Is Correlated With Infrared Spectroscopic Imaging-Derived Parameters. A. Hanifi, J. B. Richardson, J. H. Kuiper, S. Roberts, N. Pleshko Osteoarthritis Cartilage. 2012 Sep; 20(9): 988-996.
    • 7. Vasiliadis HS, Danielson B, Ljungberg M, McKeon B, Lindahl A, Peterson L. Autologous chondrocyte implantation in cartilage lesions of 21
    • 8. Sharma A, Wood LD, Richardson JB, Roberts S, Kuiper NJ. Glycosaminoglycan profiles of repair tissue formed following autologous chondrocyte implantation differ from control cartilage. Arthritis Res Ther. 2007;9(4):R79.
    • 10. Van Assche D, Staes F, Van Caspel D, Vanlauwe J, Bellemans J, Saris DB, Luyten FP. Autologous chondrocyte implantation versus microfracture for knee cartilage injury: a prospective randomized trial, with 2-year followup. Knee Surg Sports Traumatol Arthrosc. 2010;18(4):486-495.
    • 11. Jones DG, Peterson L. Autologous chondrocyte implantation. J Bone Joint Surg Am. 2006;88(11):2502-2520.
    • 12. Brun P, Dickinson SC, Zavan B, Cortivo R, Hollander AP, Abatangelo G. Characteristics of repair tissue in second-look and third-look biopsies from patients treated with engineered cartilage: relationship to symptomatology and time after implantation. Arthritis Res Ther. 2008;10(6):R132.
    • 13. Krishnan SP, Skinner JA, Bartlett W, Carrington RW, Flanagan AM, Briggs TW, Bentley G. Who is the ideal candidate for autologous chondrocyte implantation? J Bone Joint Surg Br. 2006 Jan;88(1):61-64. 22
    • 14. José Becerra, José A. Andrades, Enrique Guerado, Plácido ZamoraNavas, José M. López-Puertas and A. Hari Reddi. Articular cartilage: structure and regeneration. Tissue Engineering Part B: Reviews. 2010; 16(6): 617-627.
    • 15. Kramer K. Specific sides to multifaceted glycosaminoglycans are observed in embryonic development. Seminars in Cell & Developmental Biology 2010;21:631-637.
    • 16. Huckerby TN, Lauder RM, Brown GM, Nieduszynski IA, Anderson K, Boocock J, Sandall PL, Weeks SD. Characterization of oligosaccharides from the chondroitin sulfates. (1)H-NMR and (13)C-NMR studies of reduced disaccharides and tetrasaccharides. Eur J Biochem. 2001;268(5):1181-1189.
    • 17. Yamada S, Onishi M, Fujinawa R, Tadokoro Y, Okabayashi K, Asashima M, Sugahara K. Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis. Glycobiology. 2009;19(5):488-498.
    • 18. West LA, Roughley P, Nelson FR, Plaas AK. Sulphation heterogeneity in the trisaccharide (GalNAcSb1,4GlcAb1,3GalNAcS) isolated from the nonreducing terminal of human aggrecan chondroitin sulphate. Biochem. J. 1999;342:223-229.
    • 19. Plaas AH, West LA, Midura RJ. Keratan sulfate disaccharide composition determined by FACE analysis of keratanase II and endo-betagalactosidase digestion products. Glycobiology 2001;11(10):779-790.
    • 27. Karousou E, Asimakopoulou A, Monti L, Zafeiropoulou V, Afratis N, Gartaganis P, Rossi A, Passi A, Karamanos NK. FACE analysis as a fast and reliable methodology to monitor the sulfation and total amount of chondroitin sulfate in biological samples of clinical importance. Molecules. 2014 Jun 12;19(6):7959-7980.
    • 28. McCarthy HS, Roberts S. A histological comparison of the repair tissue formed when using either Chondrogide(®) or periosteum during autologous chondrocyte implantation. Osteoarthritis Cartilage. 2013 Dec;21(12):2048-2057.
    • 29. Roberts S, Menage J, Sandell LJ, Evans EH, Richardson JB. Immunohistochemical study of collagen types I and II and procollagen IIA in human cartilage repair tissue following autologous chondrocyte implantation. Knee. 2009 Oct;16(5):398-404.
    • 30. Niethammer TR, Limbrunner K, Betz OB, Gülecyüz MF, Pietschmann MF, Feist M, Müller PE. Int Orthop. Analysis of the autologous chondrocyte quality of matrix-based autologous chondrocyte implantation in the knee joint. International Orthopaedics (SICOT). 2016 Jan;40(1):205- 212.
    • 32. Kameda T, Koike C, Saitoh K, Kuroiwa A, Iba H. Analysis of cartilage maturation using micromass cultures of primary chondrocytes. Dev Growth Differ. 2000 Jun;42(3):229-236.
    • 33. Williams GM, Klisch SM, Sah R. Bioengineering Cartilage Growth, Maturation, and Form. Pediatric Research 2008;63:527-534.
    • 34. Bonaventure J, Kadhom N, Cohen-Solal L, Ng KH, Bourguignon J, Lasselin C, Freisinger P. Re-expression of cartilage-specific genes by redifferentiated human articular chondrocytes cultured in alginate beads. Exp Cell Res. 1994;212(1):97-104.
    • 35. Kolettas E, Buluwela L, Bayliss MT, Muir H. Expression of cartilagespecific molecules is retained on long-term culture of human articular chondrocytes. Journal of Cell Science 1995;108:1991-1999.
    • 36. Harrison PE, Ashton IK, Johnson WE, Turner SL, Richardson JB, Ashton BA. The in vitro growth of human chondrocytes. Cell Tissue Bank. 2000;1(4):255-260.
    • 37. Liu H, Lee YW, Dean MF. Re-expression of differentiated proteoglycan phenotype by dedifferentiated human chondrocytes during culture in alginate beads. Biochim Biophys Acta. 1998;1425(3):505-15.
    • 38. Maroudas A, Bayliss MT, Uchitel-Kaushansky N, Schneiderman R, Gilav E. Aggrecan Turnover in Human Articular Cartilage: Use of Aspartic
    • 39. Kobayashi S, Meir A, Urban J. Effect of cell density on the rate of glycosaminoglycan accumulation by disc and cartilage cells in vitro. J Orthop Res. 2008 Apr;26(4):493-503.
    • 40. Sun HB. Mechanical loading, cartilage degradation, and arthritis. Ann N Y Acad Sci. 2010;1211:37-50.
    • 41. Wang QG, Nguyen B, Thomas CR, Zhang Z, El Haj AJ, Kuiper NJ. Molecular profiling of single cells in response to mechanical force: comparison of chondrocytes, chondrons and encapsulated chondrocytes. Biomaterials. 2010;31(7):1619-1625.
    • 42. Brown MP, Trumble TN, Plaas AH, Sandy JD, Romano M, Hernandez J, Merritt KA. Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid. Osteoarthritis Cartilage. 2007 Nov;15(11):1318-25.
    • 43. Bailey A, Kuiper (nee Goodstone) NJ, Roberts S, Hughes J, Roberts S, Niekerk L, Richardson JB, Rees D. Rehabilitation after Oswestry autologous-chondrocyte implantation: the OsCell protocol. J Sport Rehabil. 2003;12:104-118.
    • 44. Roden L. Structure and metabolism of connective tissue proteoglycans. In: Lennarz W, Ed. The Biochemistry of Glycoproteins and Proteoglycans. New York: Plenum Press 1980:286-306.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article