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Gillis, Richard B.; Adams, Gary G.; Heinze, Thomas; Nikolajski, Melanie; Harding, Stephen E.; Rowe, Arthur J. (2013)
Publisher: Springer Nature
Journal: European Biophysics Journal
Languages: English
Types: Article
Subjects: Sedimentation equilibrium, Point average molecular weights, Original Paper, Rayleigh interference optics, Fitting algorithm, Biophysics, Spatial filtering, Molecular weight histograms
MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe) system employed, thus supplanting existing methods of analysis through reducing the ‘noise’ level of certain important parameter estimates by up to orders of magnitude. Long-known limitations of the SE method, arising from lack of knowledge of the true fringe number in fringe optics and from the use of unstable numerical algorithms such as numerical differentiation, have been transcended. An approach to data analysis, akin to ‘spatial filtering’, has been developed, and shown by both simulation and practical application to be a powerful aid to the precision with which near-monodisperse systems can be analysed, potentially yielding information on protein-solvent interaction. For oligo- and poly-disperse systems the information returned includes precise average mass distributions over both cell radial and concentration ranges and mass-frequency histograms at fixed radial positions. The application of MultiSig analysis to various complex heterogenous systems and potentially multiply-interacting carbohydrate oligomers is described.
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    • Ang S, Rowe AJ (2010) Evaluation of the information content of sedimentation equilibrium data in self-interacting systems. Macromol Biosci 10:798-807
    • Azis BH, Chin B, Deacon MP, Harding SE, Pavlov GM (1999) Size and shape of inulin in dimethyl sulphoxide solution. Carbohyd Polym 38:231-234
    • Chalikian TV (2003) Volume properties of proteins. Annu Rev Biophys Biomol Struct 32:207-235
    • Harding SE (2005) Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Carbohydr Res 340:811-826
    • Heinze T, Nikolajski M, Daus S, Besong Tabot MD, Michaelis N, Berlin P, Morris GA, Rowe AJ, Harding SE (2011) Protein-like oligomerisation of carbohydrates. Angew Chem Int Ed 50:8602-8604
    • Imran S, Gillis RB, Kok MS, Harding SE, Adams GG (2012) Application and use of inulin as a tool for therapeutic drug delivery. Biotechnol Genet Eng Revi 28:33-46
    • Kawahara K, Ohta K, Miyamoto H, Nakamura S (1984) Preparation and solution properties of pullulan fractions as standard samples for water-soluble polymers. Carbohydr Polym 4:335-356
    • Laue TM (1992) In: Harding SE, Rowe AJ, Horton JC (eds) Analytical ultracentrifugation in biochemistry and polymer science. Royal Society of Chemistry, Cambridge, pp 63-89
    • Lugt AV (1964) Signal detection by complex spatial filtering. IEEE T Inform Theory 10:139-145
    • Ockleford CD, Cairns H, Rowe AJ, Byrne S, Scott JJA, Willingale R (2002) The distribution of caveolin-3 immunofluorescence in skeletal muscle fibre membranes defined by dual channel confocal laser scanning microscopy, fast Fourier transform and image modelling. J Microsc 206:93-105
    • Petersson J, Holmstro¨m K (1998) Mathematics/Applied Mathematics. Technical Report IMa-TOM-1997-08
    • Roark DE, Yphantis DA (1969) Studies of self-associating systems by sedimentation equilibrium. Ann N Y Acad Sci 164:245-278
    • Rowe AJ (2011) Ultra-weak reversible protein-protein interactions. Methods 54:157-166
    • Rowe AJ (2004) Determination of the absolute fringe number in Rayleigh interference patterns in systems at sedimentation equilibrium via a newly defined function (kD). Progr Colloid Polym Sci 127:1-8
    • Schuck P (2000) Size distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation modeling. Biophys J 78:1606-1619
    • Teller DC (1973) Characterization of proteins by sedimentation equilibrium in the analytical ultracentrifuge. Method Enzymol 27:346-441
    • Windfield S, Jonassen TEN, Christensen S (2003) [3H]Inulin as a marker for glomerular filtration rate. Am J Physiol 285:575-576
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