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Black, Gary; Brown, Nicola; Perry, Justin; Randall, P. David; Turnbull, Graeme; Zhang, Meng (2015)
Publisher: Royal Society of Chemistry
Languages: English
Types: Article
Subjects: B200
Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.
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