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Bing, C.; Russell, S.; Becket, E.; Pope, M.; Tisdale, M.J.; Trayhurn, P.; Jenkins, J.R.
Journal: British Journal of Cancer
Languages: English
Types: Article
Subjects: Translational Therapeutics, SREBP-1c, cancer cachexia, adipose tissue, C/EBP?, mice
Extensive loss of adipose tissue is a hallmark of cancer cachexia but the cellular and molecular basis remains unclear. This study has examined morphologic and molecular characteristics of white adipose tissue in mice bearing a cachexia-inducing tumour, MAC16. Adipose tissue from tumour-bearing mice contained shrunken adipocytes that were heterogeneous in size. Increased fibrosis was evident by strong collagen-fibril staining in the tissue matrix. Ultrastructure of 'slimmed' adipocytes revealed severe delipidation and modifications in cell membrane conformation. There were major reductions in mRNA levels of adipogenic transcription factors including CCAAT/enhancer binding protein alpha (C/EBPα), CCAAT/enhancer binding protein beta, peroxisome proliferator-activated receptor gamma, and sterol regulatory element binding protein-1c (SREBP-1c) in adipose tissue, which was accompanied by reduced protein content of C/EBPα and SREBP-1. mRNA levels of SREBP-1c targets, fatty acid synthase, acetyl CoA carboxylase, stearoyl CoA desaturase 1 and glycerol-3-phosphate acyl transferase, also fell as did glucose transporter-4 and leptin. In contrast, mRNA levels of peroxisome proliferators-activated receptor gamma coactivator-1alpha and uncoupling protein-2 were increased in white fat of tumour-bearing mice. These results suggest that the tumour-induced impairment in the formation and lipid storing capacity of adipose tissue occurs in mice with cancer cachexia. © 2006 Cancer Research UK.
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    • Argiles JM, Moore-Carrasco R, Fuster G, Busquets S, Lopez-Soriano FJ (2003) Cancer cachexia: the molecular mechanisms. Int J Biochem Cell Biol 35: 405 - 409
    • Bastard JP, Caron M, Vidal H, Jan V, Auclair M, Vigouroux C, Luboinski J, Laville M, Maachi M, Girard PM, Rozenbaum W, Levan P, Capeau J (2002) Association between altered expression of adipogenic factor SREBP1 in lipoatrophic adipose tissue from HIV-1-infected patients and abnormal adipocyte differentiation and insulin resistance. Lancet 359: 1026 - 1031
    • Bibby MC, Double JA, Ali SA, Fearon KC, Brennan RA, Tisdale MJ (1987) Characterization of a transplantable adenocarcinoma of the mouse colon producing cachexia in recipient animals. J Natl Cancer Inst 78: 539 - 546
    • Bing C, Bao Y, Jenkins J, Sanders P, Manieri M, Cinti S, Tisdale MJ, Trayhurn P (2004) Zinc-alpha2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia. Proc Natl Acad Sci USA 101: 2500 - 2505
    • Bing C, Brown M, King P, Collins P, Tisdale MJ, Williams G (2000) Increased gene expression of brown fat uncoupling protein (UCP)1 and skeletal muscle UCP2 and UCP3 in MAC16-induced cancer cachexia. Cancer Res 60: 2405 - 2410
    • Bing C, Taylor S, Tisdale MJ, Williams G (2001) Cachexia in MAC16 adenocarcinoma: suppression of hunger despite normal regulation of leptin, insulin and hypothalamic neuropeptide Y. J Neurochem 79: 1004 - 1012
    • Fajas L, Schoonjans K, Gelman L, Kim JB, Najib J, Martin G, Fruchart JC, Briggs M, Spiegelman BM, Auwerx J (1999) Regulation of peroxisome proliferator-activated receptor gamma expression by adipocyte differentiation and determination factor 1/sterol regulatory element binding protein 1: implications for adipocyte differentiation and metabolism. Mol Cell Biol 19: 5495 - 5503
    • Hale LP, Price DT, Sanchez LM, Demark-Wahnefried W, Madden JF (2001) Zinc alpha-2-glycoprotein is expressed by malignant prostatic epithelium and may serve as a potential serum marker for prostate cancer. Clin Cancer Res 7: 846 - 853
    • Horton JD (2002) Sterol regulatory element-binding proteins: transcriptional activators of lipid synthesis. Biochem Soc Trans 30: 1091 - 1095
    • Jan V, Cervera P, Maachi M, Baudrimont M, Kim M, Vidal H, Girard PM, Levan P, Rozenbaum W, Lombes A, Capeau J, Bastard JP (2004) Altered fat differentiation and adipocytokine expression are inter-related and linked to morphological changes and insulin resistance in HIV-1- infected lipodystrophic patients. Antivir Ther 9: 555 - 564
    • Kakuma T, Wang ZW, Pan W, Unger RH, Zhou YT (2000) Role of leptin in peroxisome proliferator-activated receptor gamma coactivator-1 expression. Endocrinology 141: 4576 - 4582
    • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402 - 408
    • Lopez-Soriano J, Argiles JM, Lopez-Soriano FJ (1997a) Sequential changes in lipoprotein lipase activity and lipaemia induced by the Yoshida AH130 ascites hepatoma in rats. Cancer Lett 116: 159 - 165
    • Lopez-Soriano J, Lopez-Soriano FJ, Bagby GJ, Williamson DH, Argiles JM (1997b) Anti-TNF treatment does not reverse the abnormalities in lipid metabolism of the obese Zucker rat. Am J Physiol 272: E656 - E660
    • Machado AP, Costa Rosa LF, Seelaender MC (2004) Adipose tissue in Walker 256 tumour-induced cachexia: possible association between decreased leptin concentration and mononuclear cell infiltration. Cell Tissue Res 318: 503 - 514
    • Mandrup S, Lane MD (1997) Regulating adipogenesis. J Biol Chem 272: 5367 - 5370
    • Orci L, Cook WS, Ravazzola M, Wang MY, Park BH, Montesano R, Unger RH (2004) Rapid transformation of white adipocytes into fat-oxidizing machines. Proc Natl Acad Sci USA 101: 2058 - 2063
    • Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM (1998) A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell 92: 829 - 839
    • Rofe AM, Bourgeois CS, Coyle P, Taylor A, Abdi EA (1994) Altered insulin response to glucose in weight-losing cancer patients. Anticancer Res 14: 647 - 650
    • Rosen ED (2005) The transcriptional basis of adipocyte development. Prostaglandins Leukot Essent Fatty Acids 73: 31 - 34
    • Russell ST, Tisdale MJ (2002) Effect of a tumour-derived lipid-mobilising factor on glucose and lipid metabolism in vivo. Br J Cancer 87: 580 - 584
    • Semple RK, Crowley VC, Sewter CP, Laudes M, Christodoulides C, Considine RV, Vidal-Puig A, O'Rahilly S (2004) Expression of the thermogenic nuclear hormone receptor coactivator PGC-1alpha is reduced in the adipose tissue of morbidly obese subjects. Int J Obes Relat Metab Disord 28: 176 - 179
    • Shimano H, Yahagi N, Amemiya-Kudo M, Hasty AH, Osuga J, Tamura Y, Shionoiri F, Iizuka Y, Ohashi K, Harada K, Gotoda T, Ishibashi S, Yamada N (1999) Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. J Biol Chem 274: 35832 - 35839
    • Simons PJ, van den Pangaart PS, van Roomen CP, Aerts JM, Boon L (2005) Cytokine-mediated modulation of leptin and adiponectin secretion during in vitro adipogenesis: evidence that tumor necrosis factor-alphaand interleukin-1beta-treated human preadipocytes are potent leptin producers. Cytokine 32: 94 - 103
    • Thompson MP, Cooper ST, Parry BR, Tuckey JA (1993) Increased expression of the mRNA for hormone-sensitive lipase in adipose tissue of cancer patients. Biochim Biophys Acta 1180: 236 - 242
    • Thompson MP, Kim D (2004) Links between fatty acids and expression of UCP2 and UCP3 mRNAs. FEBS Lett 568: 4 - 9
    • Thompson MP, Koons JE, Tan ET, Grigor MR (1981) Modified lipoprotein lipase activities, rates of lipogenesis, and lipolysis as factors leading to lipid depletion in C57BL mice bearing the preputial gland tumor, ESR586. Cancer Res 41: 3228 - 3232
    • Tiraby C, Tavernier G, Lefort C, Larrouy D, Bouillaud F, Ricquier D, Langin D (2003) Acquirement of brown fat cell features by human white adipocytes. J Biol Chem 278: 33370 - 33376
    • Tisdale MJ (2002) Cachexia in cancer patients. Nat Rev Cancer 2: 862 - 871
    • Todorov PT, McDevitt TM, Meyer DJ, Ueyama H, Ohkubo I, Tisdale MJ (1998) Purification and characterization of a tumor lipid-mobilizing factor. Cancer Res 58: 2353 - 2358
    • Trayhurn P, Bing C (2006) Appetite and energy balance signals from adipocytes. Philos Trans R Soc Lond B Biol Sci 361: 1237 - 1249
    • Wang ND, Finegold MJ, Bradley A, Ou CN, Abdelsayed SV, Wilde MD, Taylor LR, Wilson DR, Darlington GJ (1995) Impaired energy homeostasis in C/EBP alpha knockout mice. Science 269: 1108 - 1112
    • Wu Z, Rosen ED, Brun R, Hauser S, Adelmant G, Troy AE, McKeon C, Darlington GJ, Spiegelman BM (1999) Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell 3: 151 - 158
    • Yoshikawa T, Noguchi Y, Satoh S, Doi C, Okamoto T, Nomura K, Makino T, Tsuburaya A, Matsumoto A (1997) Insulin resistance and the alterations of glucose transporter-4 in adipose cells from cachectic tumor-bearing rats. JPEN J Parenter Enteral Nutr 21: 347 - 349
    • Zhang B, Berger J, Hu E, Szalkowski D, White-Carrington S, Spiegelman BM, Moller DE (1996) Negative regulation of peroxisome proliferator-activated receptor-gamma gene expression contributes to the antiadipogenic effects of tumor necrosis factor-alpha. Mol Endocrinol 10: 1457 - 1466
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