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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Azizi, Armaghan
Languages: English
Types: Doctoral thesis
Subjects: UOW2
Pierce's Disease (PD) is one of the most devastating diseases threatening grapevines\ud in the USA caused by the Gram negative bacterium Xylella fastidiosa. There is no\ud effective control method for Pierce’s Disease and control is mainly achieved by\ud suppressing transmission of the glassy winged sharpshooter (GWSS) insect vector.\ud The study of the surface proteins of X. fastidiosa may provide targets that may be\ud attractive sites for intervention. MopB is an outer membrane protein, which has been\ud identified on the X. fastidiosa surface. Blocking MopB with antibodies may prevent\ud or reduce transmission of the pathogen.\ud The main goal of this research was to generate recombinant antibodies to be used to\ud investigate the interaction of MopB with the plant and GWSS. Towards this goal,\ud recombinant MopB has been produced and used with sera from five mice immunized\ud with whole heat killed X. fastidiosa in an Enzyme-Linked Immunosorbent assay\ud (ELISA). The ELISA results revealed the presence of antibodies against the\ud recombinant MopB protein. In vitro combinatorial antibody ribosome display\ud libraries were assembled from immunoglobulin transcripts rescued from the spleens\ud of mice immunized with heat-killed X. fastidiosa. The libraries were used in a single\ud round of selection against the outer-membrane protein MopB, resulting in the\ud isolation of a panel of recombinant antibodies. The potential use of selected anti-\ud MopB antibodies was demonstrated by the successful application of the 4XfMopB3\ud antibody in ELISA, Western blot and immunofluorescence assays.\ud Pantoea agglomerans, a candidate bacterium for PD paratransgenic approach was\ud engineered to express and secrete selected anti-MopB (4XfMopB3) single chain\ud antibody. The expression cassette that include the pelB secretion signal was created\ud and tested for its efficiency\ud in secreting the 4XfMopB3 antibody in E. coli and\ud P. agglomerans. The 4XfMopB3 antibody was successfully expressed and secreted in\ud E. coli while it was only expressed in P. agglomerans.
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    • Table 2.1 Primers (5' to 3' direction) used in PCR. ...............................................................................42  
    • Table 3.1 Total RNA extraction concentrations and A260/280 and A260/230 ratios obtained from 5 mice spleens previously immunized with whole heat-killed X. fastidiosa. ...........................................83  
    • Table 3.2 Junction analysis and alignment of the VL and VH CDR3 sequences of the selected antiMopB scFv's (4XfMopB1-4). ......................................................................................................88  
    • Table 3.3 Pantoea agglomerans antibiogram zones of inhibition.........................................................99   HE, M. & TAUSSIG, M. J. 2002. Ribosome display: cell-free protein display technology. Briefings in Functional Genomics and Proteomics, 1, 204-212.
    • HE, M. & TAUSSIG, M. J. 2007. Eukaryotic ribosome display with in situ DNA recovery. Nature Methods, 4, 281-288.
    • HE, M. Y. & KHAN, F. 2005. Ribosome display: next-generation display technologies for production of antibodies in vitro. Expert Review of Proteomics, 2, 421-430.
    • HEARON, S. S., SHERALD, J. L. & KOSTKA, S. J. 1980. Association of xylemlimited bacteria with elm, sycamore, and oak leaf scorch. Canadian Journal of Botany-Revue Canadienne De Botanique, 58, 1986-1993.
    • HENNEBERGER, T. S. M., STEVENSON, K. L., BRITTON, K. O. & CHANG, C. J. 2004. Distribution of Xylella fastidiosa in sycamore associated with low temperature and host resistance. Plant Disease, 88, 951-958.
    • HEWITT, W. B., HOUSTON, B. R., FRAZIER, N. W. & FREITAG, J. H. 1946. Leafhopper transmission of the virus causing Pierces Disease of grape and dwarf of alfalfa. Phytopathology, 36, 117-128.
    • HILL, B. L. & PURCELL, A. H. 1995. Multiplication and movement of Xylella fastidiosa within grapevine and 4 other plants. Phytopathology, 85, 1368- 1372.
    • HOPKINS, D. L. 1989. Xylella fastidiosa - xylem-limited bacterial pathogen of plants. Annual Review of Phytopathology, 27, 271-290.
    • HOPKINS, D. L. 2005. Biological control of Pierce's Disease in the vineyard with strains of Xylella fastidiosa benign to grapevine. Plant Disease, 89, 1348- 1352.
    • HOPKINS, D. L. & ADLERZ, W. C. 1988. Natural hosts of Xylella fastidiosa in florida. Plant Disease, 72, 429-431.
    • HOPKINS, D. L. & MOLLENHA, HH. 1973. Rickettsia-like bacterium associated with Pierces Disease of grapes. Science, 179, 298-300.
    • HOPKINS, D. L. & MORTENSE, JA. 1971. Suppression of pierces disease symptoms by tetracycline antibiotics. Plant Disease Reporter, 55, 610-612.
    • HOPKINS, D. L. & PURCELL, A. H. 2002. Xylella fastidiosa: Cause of Pierce's disease of grapevine and other emergent diseases. Plant Disease, 86, 1056- 1066.
    • HUSTON, J. S., MUDGETTHUNTER, M., TAI, M. S., MCCARTNEY, J., WARREN, F., HABER, E. & OPPERMANN, H. 1991. Protein engineering of single-chain fv analogs and fusion proteins. Methods in Enzymology, 203, 46-88.
    • HUTCHINS, L. M., COCHRAN, L. C., TURNER, W. F. & WEINBERGER, J. H. 1953. Transmission of phony disease virus from tops of certain affected peach and plum trees. Phytopathology, 43, 691-696.
    • JACKSON, B. C., BLUA, M. J. & BEXTINE, B. 2008. Impact of duration versus frequency of probing by Homalodisca vitripennis (Hemiptera : Cicadellidae) on inoculation of Xylella fastidiosa. Journal of Economic Entomology, 101, 1122-1126.
    • KANE, J. F. 1995. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Current Opinion in Biotechnology, 6, 494-500.
    • KANGA, L. H. B., JONES, W. A., HUMBER, R. A. & BOYD, D. W. JR. 2004. Fungal pathogens of the glassy-winged sharpshooter Homalodisca coagulata (Homoptera: Cicadellidae). Florida Entomologist, 87, 225-228.
    • KETTLEBOROUGH, C. A., SALDANHA, J., ANSELL, K. H. & BENDIG, M. M. 1993. Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain-reaction. European Journal of Immunology, 23, 206-211.
    • KHAN, N. A., SHIN, S., CHUNG, J. W., KIM, K. J., ELLIOTT, S., WANG, Y. & KIM, K. S. 2003. Outer membrane protein A and cytotoxic necrotizing factor1 use diverse signaling mechanisms for Escherichia coli K1 invasion of human brain microvascular endothelial cells. Microbial Pathogenesis, 35, 35- 42.
    • KIPRIYANOV, S. M. 2002. High-level periplasmic expression and purification of scFvs. Methods in molecular biology (Clifton, N.J.), 178, 333-41.
    • KOEBNIK, R., LOCHER, K. P. & VAN GELDER, P. 2000. Structure and function of bacterial outer membrane proteins: barrels in a nutshell. Molecular Microbiology, 37, 239-253.
    • KOHLER, G. & MILSTEIN, C. 1975. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, 256, 495-497.
    • KOIDE, T., VENCIO, R. Z. N. & GOMES, S. L. 2006. Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa. Journal of Bacteriology, 188, 5821-5830.
    • KOLAJ, O., SPADA, S., ROBIN, S. & WALL, J. G. 2009. Use of folding modulators to improve heterologous protein production in Escherichia coli. Microbial Cell Factories, 8, 9-25.
    • KOST, T. A., CONDREAY, J. P. & JARVIS, D. L. 2005. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nature Biotechnology, 23, 567-575.
    • KOSTAKIOTI, M., NEWMAN, C. L., THANASSI, D. G. & STATHOPOULOS, C. 2005. Mechanisms of protein export across the bacterial outer membrane. Journal of Bacteriology, 187, 4306-4314.
    • KOZAK, M. 1987. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research, 15, 8125-8148.
    • KREWER, G., DUTCHER, J. D. & CHANG, C. J. 2002. Imidacloprid insecticide slows development of Pierce's Disease in bunch grapes. Journal of Entomological Science, 37, 101-112.
    • KROGH, A. 1997. Two methods for improving performance of an HMM and their application for gene finding. Proceedings / ... International Conference on Intelligent Systems for Molecular Biology ; ISMB. International Conference on Intelligent Systems for Molecular Biology, 5, 179-186.
    • KRUGNER, R., JOHNSON, M. W., GROVES, R. L. & MORSE, J. G. 2008. Host specificity of Anagrus epos: a potential biological control agent of Homalodisca vitripennis. Biocontrol, 53, 439-449.
    • KURLAND, C. & GALLANT, J. 1996. Errors of heterologous protein expression. Current Opinion in Biotechnology, 7, 489-493.
    • KUZINA, L. V., MILLER, T. A. & COOKSEY, D. A. 2006. In vitro activities of antibiotics and antimicrobial peptides against the plant pathogenic bacterium Xylella fastidiosa. Letters in Applied Microbiology, 42, 514-520.
    • LACAVAL, P. T., AZEVEDO, J. L., MILLER, T. A. & S., H. J. 2009. Interactions of Xylella fastidiosa and Endophytic Bacteria in Citrus: A Review. Tree and Forestry Science and Biotechnology, Global Science Books, 40-46.
    • LAEMMLI, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-5.
    • LAMPE, D. J., R., C., LAUZON, C. R. & MILLER, T. 2006. Development of symbiotic control of Pierce's Disease. http://biopesticide.ucr.edu/abstracts/assets/Lampe_abstract.pdf.
    • LEFRANC, M. P., GIUDICELLI, V., GINESTOUX, C., JABADO-MICHALOUD, J., FOLCH, G., BELLAHCENE, F., WU, Y., GEMROT, E., BROCHET, X., LANE, J., REGNIER, L., EHRENMANN, F., LEFRANC, G. & DUROUX, P. 2009. IMGT (R), the international ImMunoGeneTics information system (R). Nucleic Acids Research, 37, D1006-D1012.
    • LEI, S. P., LIN, H. C., WANG, S. S., CALLAWAY, J. & WILCOX, G. 1987. Characterization of the Erwinia carotovora pelB gene and its product pectate lyase. Journal of Bacteriology, 169, 4379-4383.
    • LI, Y. X., HAO, G. X., GALVANI, C. D., MENG, Y. Z., DE LA FUENTE, L., HOCH, H. C. & BURR, T. J. 2007. Type I and type IV pili of Xylella LINDOW, S. E. & BRANDL, M. T. 2003. Microbiology of the phyllosphere. Applied and Environmental Microbiology, 69, 1875-1883.
    • LONCARIC, I., HEIGL, H., LICEK, E., MOOSBECKHOFER, R., BUSSE, H. J. & ROSENGARTEN, R. 2009. Typing of Pantoea agglomerans isolated from colonies of honey bees (Apis mellifera) and culturability of selected strains from honey. Apidologie, 40, 40-54.
    • MAKRIDES, S. C. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiological Reviews, 60, 512-38.
    • MARKIV, A., ANANI, B., DURVASULA, R. V. & KANG, A. S. 2011. Module based antibody engineering: A novel synthetic REDantibody. Journal of Immunological Methods, 364, 40-49.
    • MARTIN, C. D., ROJAS, G., MITCHELL, J. N., VINCENT, K. J., WU, J., MCCAFFERTY, J. & SCHOFIELD, D. J. 2006. A simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnology, 6, 46-60.
    • MARTINEAU, P. & BETTON, J. M. 1999. In vitro folding and thermodynamic stability of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm. Journal of Molecular Biology, 292, 921-929.
    • MATTHEAKIS, L. C., BHATT, R. R. & DOWER, W. J. 1994. An in vitro polysome display system for identifying ligands from very large peptide libraries. Proceedings of the National Academy of Sciences of the United States of America, 91, 9022-9026.
    • MATTHEWS, S., RAO, V. S. & DURVASULA, R. V. 2011. Modeling horizontal gene transfer (HGT) in the gut of the Chagas disease vector Rhodnius prolixus. Parasites & Vectors, 4, 77-85.
    • MENG, Y. Z., LI, Y. X., GALVANI, C. D., HAO, G. X., TURNER, J. N., BURR, T. J. & HOCH, H. C. 2005. Upstream migration of Xylella fastidiosa via pilusdriven twitching motility. Journal of Bacteriology, 187, 5560-5567.
    • MEYER, T. S. & LAMBERTS, B. L. 1965. Use of coomassie brilliant blue R250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide-gel strips. Biochim Biophys Acta, 107, 144-145.
    • MILLER, T. 2007. Symbiotic control applications in agriculture and medicine. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 146, S218-S218.
    • MILLER, T. A. 2011. Paratransgenesis as a potential tool for pest control: review of applied arthropod symbiosis. Journal of Applied Entomology, 135, 474-478.
    • MINSAVAGE, G. V., THOMPSON, C. M., HOPKINS, D. L., LEITE, R. & STALL, R. E. 1994. Development of a polymerase chain-reaction protocol for detection of Xylella fastidiosa in plant-tissue. Phytopathology, 84, 456-461.
    • MIRCETICH, S. M., LOWE, S. K., MOLLER, W. J. & NYLAND, G. 1976. Etiology of almond leaf scorch disease and transmission of causal agent. Phytopathology, 66, 17-24.
    • MORANO, L. D., BEXTINE, B. R., GARCIA, D. A., MADDOX, S. V., GUNAWAN, S., VITOVSKY, N. J. & BLACK, M. C. 2008. Initial genetic analysis of Xylella fastidiosa in Texas. Current Microbiology, 56, 346-351.
    • MORTENSEN, J. A., STOVER, L. H. & BALERDI, C. F. 1977. Sources of resistance to pierces disease in vitis. Journal of the American Society for Horticultural Science, 102, 695-697.
    • MYERS, A. L., SUTTON, T. B., ABAD, J. A. & KENNEDY, G. G. 2007. Pierce's Disease of grapevines: Identification of the primary vectors in North Carolina. Phytopathology, 97, 1440-1450.
    • NADARASAH, G. & STAVRINIDES, J. 2011. Insects as alternative hosts for phytopathogenic bacteria. FEMS Microbiology Reviews, 35, 555-575.
    • NOVY, R., DROTT, D., YAEGER, K. & MIERENDORF, R. 2001. Overcoming the codon bias of E. coli for enhanced protein expression. InNovations (Novagen, Inc. newsletter), 12, 1-3.
    • NYLAND, G., GOHEEN, A. C., LOWE, S. K. & KIRKPATR.HC 1973. Ultrastructure of a rickettsia-like organism from a peach tree affected with phony disease. Phytopathology, 63, 1275-1278.
    • O'TOOLE, G. A., PRATT, L. A., WATNICK, P. I., NEWMAN, D. K., WEAVER, V. B. & KOLTER, R. 1999. Genetic approaches to study of biofilms. Methods in Enzymology, 310, 91-109.
    • OLIVEIRA, A. C., VALLIM, M. A., SEMIGHINI, C. P., ARAUJO, W. L., GOLDMAN, G. H. & MACHADO, M. A. 2002. Quantification of Xylella fastidiosa from citrus trees by real-time polymerase chain reaction assay. Phytopathology, 92, 1048-1054.
    • PEREZ-DONOSO, A. G., GREVE, L. C., WALTON, J. H., SHACKEL, K. A. & LABAVITCH, J. M. 2007. Xylella fastidiosa infection and ethylene exposure result in xylem and water movement disruption in grapevine shoots. Plant Physiology, 143, 1024-1036.
    • PEREZ-DONOSO, A. G., SUN, Q., ROPER, M. C., GREVE, L. C., KIRKPATRICK, B. & LABAVITCH, J. M. 2010. Cell wall-degrading enzymes enlarge the pore size of intervessel pit membranes in healthy and Xylella fastidiosa infected grapevines. Plant Physiology, 152, 1748-1759.
    • PILKINGTON, L. J., IRVIN, N. A., BOYD, E. A., HODDLE, M. S., TRIAPITSYN, S. V., CAREY, B. G., JONES, W. A. & MORGAN, D. J. W. 2005. Introduced parasitic wasps could control glassy-winged sharpshooter. California Agriculture (REVIEW ARTICLE), 59, 223- 228.
    • PLUECKTHUN, A. 1991. Strategies for the expression of antibody fragments in Escherichia coli. Methods (Orlando), 2, 88-96.
    • PRASADARAO, N. V., WASS, C. A., WEISER, J. N., STINS, M. F., HUANG, S. H. & KIM, K. S. 1996. Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells. Infection and Immunity, 64, 146-153.
    • PRINZ, W. A., ASLUND, F., HOLMGREN, A. & BECKWITH, J. 1997. The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm. Journal of Biological Chemistry, 272, 15661-15667.
    • PURCELL, A. H., FINLAY, A. H. & MCLEAN, D. L. 1979. Pierces Disease bacterium mechanism of transmission by leafhopper vectors. Science, 206, 839-841.
    • PURCELL, A. H. & HOPKINS, D. L. 1996. Fastidious xylem-limited bacterial plant pathogens. Annual Review of Phytopathology, 34, 131-151.
    • PURCELL, A. H., SAUNDERS, S. R., HENDSON, M., GREBUS, M. E. & HENRY, M. J. 1999. Causal role of Xylella fastidiosa in oleander leaf scorch disease. Phytopathology, 89, 53-58.
    • PUSEY, P. L. 2002. Biological control agents for fire blight of apple compared under conditions limiting natural dispersal. Plant Disease, 86, 639-644.
    • RABINER, L. R. 1989. A tutorial on Hidden Markov Models and selected applications in speech recognition. Proceedings of the Ieee, 77, 257-286.
    • RAJU, B. C. & GOHEEN, A. C. 1981. Relative sensitivity of selected grapevine cultivars to Pierces Disease bacterial inoculations. American Journal of Enology and Viticulture, 32, 155-158.
    • RAJU, B. C., GOHEEN, A. C. & FRAZIER, N. W. 1983. Occurrence of Pierces Disease bacteria in plants and vectors in california. Phytopathology, 73, 1309- 1313.
    • RAJU, B. C., WELLS, J. M., NYLAND, G., BRLANSKY, R. H. & LOWE, S. K. 1982. Plum leaf scald - isolation, culture, and pathogenicity of the causal agent. Phytopathology, 72, 1460-1466.
    • RAMIREZ, J. L., LACAVA, P. T. & MILLER, T. A. 2008. Detection of the bacterium, Xylella fastidiosa, in saliva of glassy-winged sharpshooter, Homalodisca vitripennis. Journal of Insect Science, 8, 34-40.
    • RAO, S., HU, S., MCHUGH, L., LUEDERS, K., HENRY, K., ZHAO, Q., FEKETE, R. A., KAR, S., ADHYA, S. & HAMER, D. H. 2005. Toward a live microbial microbicide for HIV: Commensal bacteria secreting an HIV fusion inhibitor peptide. Proceedings of the National Academy of Sciences of the United States of America, 102, 11993-11998.
    • REN, X. X., HOICZYK, E. & RASGON, J. L. 2008. Viral paratransgenesis in the malaria vector Anopheles gambiae. Plos Pathogens, 4, e1000135-e1000141.
    • RIO, R. V. M., HU, Y. J. & AKSOY, S. 2004. Strategies of the home-team: symbioses exploited for vector-borne disease control. Trends in Microbiology, 12, 325-336.
    • RITZ, D. & BECKWITH, J. 2001. Roles of thiol-redox pathways in bacteria. Annual Review of Microbiology, 55, 21-48.
    • ROBERTS, R. W. & SZOSTAK, J. W. 1997. RNA-peptide fusions for the in vitro selection of peptides and proteins. Proceedings of the National Academy of Sciences of the United States of America, 94, 12297-12302.
    • RODRIGUES, C. M., TAKITA, M. A., COLETTA-FILHO, H. D., OLIVATO, J. C., CASERTA, R., MACHADO, M. A. & DE SOUZA, A. A. 2008. Copper resistance of biofilm cells of the plant pathogen Xylella fastidiosa. Applied Microbiology and Biotechnology, 77, 1145-1157.
    • ROPER, M. C., GREVE, L. C., LABAVITCH, J. A. & KIRKPATRICK, B. C. 2007. Detection and visualization of an exopolysaccharide produced by Xylella fastidiosa in vitro and in planta. Applied and Environmental Microbiology, 73, 7252-7258.
    • ROSSER, M. P., XIA, W., HARTSELL, S., MCCAMAN, M., ZHU, Y., WANG, S. J., HARVEY, S., BRINGMANN, P. & COBB, R. R. 2005. Transient transfection of CHO-K1-S using serum-free medium in suspension: a rapid mammalian protein expression system. Protein Expression and Purification, 40, 237-243.
    • SANCHEZ, L., AYALA, M., FREYRE, F., PEDROSO, I., BELL, H., FALCON, V. & GAVILONDO, J. V. 1999. High cytoplasmic expression in E coli, purification, and in vitro refolding of a single chain Fv antibody fragment against the hepatitis B surface antigen. Journal of Biotechnology, 72, 13-20.
    • SANDKVIST, M. 2001. Biology of type II secretion. Molecular Microbiology, 40, 271-283.
    • SCHLAPSCHY, M., GRIMM, S. & SKERRA, A. 2006. A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory SEIDEL, H. M., POMPLIANO, D. L. & KNOWLES, J. R. 1992. Phosphonate biosynthesis - molecular-cloning of the gene for phosphoenolpyruvate mutase from tetrahymena-pyriformis and overexpression of the gene-product in Escherichia coli. Biochemistry, 31, 2598-2608.
    • SHERALD, J., HEARON, S., KOSTKA, S. & MORGAN, D. 1982. Pathogenicity of a pierces disease (pd)-like bacterium cultured from leaf scorch affected sycamores. Phytopathology, 72, 710-710.
    • SHERALD, J. L. & LEI, J. D. 1991. Evaluation of a rapid elisa test kit for detection of Xylella fastidiosa in landscape trees. Plant Disease, 75, 200-203.
    • SHERALD, J. L., WELLS, J. M. & HURTT, S. S. 1987. Association of fastidious, xylem-inhabiting bacteria with leaf scorch in red maple. Plant Disease, 71, 930-933.
    • SHUSTA, E. V., VANANTWERP, J. & WITTRUP, K. D. 1999. Biosynthetic polypeptide libraries. Current Opinion in Biotechnology, 10, 117-122.
    • SPYROPOULOS, I. C., LIAKOPOULOS, T. D., BAGOS, P. G. & HAMODRAKAS, S. J. 2004. TMRPres2D: high quality visual representation of transmembrane protein models. Bioinformatics, 20, 3258-3260.
    • STEIDLER, L. 2001. Lactococcus lactis, A Tool for the Delivery of Therapeutic Proteins Treatment of IBD. Scientific World Journal, 1, 216-217.
    • STEIDLER, L., HANS, W., SCHOTTE, L., NEIRYNCK, S., OBERMEIER, F., FALK, W., FIERS, W. & REMAUT, E. 2000. Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science, 289, 1352-1355.
    • STEVENSON, J. F., MATTHEWS, M. A., GREVE, L. C., LABAVITCH, J. M. & ROST, T. L. 2004. Grapevine susceptibility to Pierce's Disease II: Progression of anatomical symptoms. American Journal of Enology and Viticulture, 55, 238-245.
    • STEVENSON, J. F., MATTHEWS, M. A. & ROST, T. L. 2005. The developmental anatomy of Pierce's Disease symptoms in grapevines: Green islands and matchsticks. Plant Disease, 89, 543-548.
    • TORCHIN, M. E., LAFFERTY, K. D., DOBSON, A. P., MCKENZIE, V. J. & KURIS, A. M. 2003. Introduced species and their missing parasites. Nature, 421, 628-630.
    • TOWBIN, H., STAEHELIN, T. & GORDON, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America, 76, 4350-4354.
    • TRIAPITSYN, S. V., & PHILLIPS, P. A. 2000. First record of Gonatocerus triguttatus (Hymenoptera: Mymaridae) from eggs of Homalodisca coagulata (Homoptera: Cicadellidae) with notes on the distribution of the host. Florida Entomologist, 83, 200-203.
    • TSUMOTO, K., EJIMA, D., KUMAGAI, I. & ARAKAWA, T. 2003. Practical considerations in refolding proteins from inclusion bodies. Protein Expression and Purification, 28, 1-8.
    • VAN EMDEN, H. F. 1989. Pest control, 2nd Edition, Edward Arnold, London, 117pp.
    • VOEGEL, T. M., WARREN, J. G., MATSUMOTO, A., IGO, M. M. & KIRKPATRICK, B. C. 2010. Localization and characterization of Xylella fastidiosa haemagglutinin adhesins. Microbiology-Sgm, 156, 2172-2179.
    • WANG, Y. 2002. The function of OmpA in Escherichia coli. Biochemical and Biophysical Research Communications, 292, 396-401.
    • WAYADANDE, A., BRUTON, B., FLETCHER, J., PAIR, S. & MITCHELL, F. 2005. Retention of cucurbit yellow vine disease bacterium Serratia marcescens through transstadial molt of vector Anasa tristis (Hemiptera : Coreidae). Annals of the Entomological Society of America, 98, 770-774.
    • WELLS, J. M., RAJU, B. C., HUNG, H. Y., WEISBURG, W. G., MANDELCOPAUL, L. & BRENNER, D. J. 1987. Xylella fastidiosa gennov, sp-nov - gram-negative, xylem-limited, fastidious plant bacteria related to Xanthomonas-spp. International Journal of Systematic Bacteriology, 37, 136-143.
    • WELLS, J. M., RAJU, B. C. & NYLAND, G. 1983. Isolation, culture, and pathogenicity of the bacterium causing phony disease of peach. Phytopathology, 73, 859-862.
    • WELLS, J. M., RAJU, B. C., THOMPSON, J. M. & LOWE, S. K. 1981. Etiology of phony peach and plum leaf scald diseases. Phytopathology, 71, 1156-1161.
    • WINTER, G., GRIFFITHS, A. D., HAWKINS, R. E. & HOOGENBOOM, H. R. 1994. Making antibodies by phage display technology. Annual Review of Immunology, 12, 433-455.
    • WOODRUFF, W. A. & HANCOCK, R. E. W. 1989. Pseudomonas aeruginosa outermembrane protein-f - structural role and relationship to the Escherichia coli ompa protein. Journal of Bacteriology, 171, 3304-3309.
    • YAN, X. H. & XU, Z. R. 2006. Ribosome-display technology: applications for directed evolution of functional proteins. Drug Discovery Today, 11, 911-916.
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