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Duck, J.M.; Young, J.P.W.; Vandenkoornhuyse, P.; Husband, R.; Daniell, T.J.; Watson, I.J.; Fitter, A.H. (2002)
Languages: English
Types: Article
Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing about two-thirds of land plant species and found in all ecosystems. They are of major importance in plant nutrient supply and their diversity is suggested to be an important determinant of plant community composition. The diversity of the AM fungal community composition in the roots of two plant species (Agrostis capillaris and Trifolium repens) that co-occurred in the same grassland ecosystem was characterized using molecular techniques. We analysed the small subunit (SSU) ribosomal RNA gene amplified from a total root DNA extract using AM fungal-specific primers. A total of 2001 cloned fragments from 47 root samples obtained on four dates were analysed by restriction fragment length polymorphism, and 121 of them were sequenced. The diversity found was high: a total of 24 different phylotypes (groups of phylogenetically related sequences) colonized the roots of the two host species. Phylogenetic analyses demonstrate that 19 of these phylotypes belonged to the Glomaceae, three to the Acaulosporaceae and two to the Gigasporaceae. Our study reveals clearly that the AM fungal community colonizing T. repens differed from that colonizing A. capillaris, providing evidence for AM fungal host preference. In addition, our results reveal dynamic changes in the AM fungal community through time.
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    • Bever JD (1999) Dynamics within mutualism and the maintenance of diversity: inference from a model of interguild frequency dependence. Ecology Letters, 2, 52 - 61.
    • Bever JD, Morton JB, Antonovics J, Schultz PA (1996) Hostdependent sporulation and species diversity of arbuscular mycorrhizal fungi in a mown grassland. Journal of Ecology, 84, 71 - 82.
    • Cavalier-Smith T, Allsopp MTEP (1996) Corallochytrium, an enigmatic non-flagellate protozoan related to choanoflagellates. European Journal of Protistology, 32, 306 - 310.
    • Clapp JP, Fitter AH, Young JPW (1999) Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology, 8, 915 - 921.
    • Clapp JP, Helgason T, Daniell TJ, Young JPW (2002) Genetic studies of the structure and diversity of arbuscular mycorrhizal fungal communities. In: Mycorrhizal Ecology (eds van der Heijden MGA, Sanders IR), pp. 201-224. Springer, Heidelberg.
    • Daniell TJ, Husband R, Fitter AH, Young JPW (2001) Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops. FEMS Microbiology Ecology, 36, 203 - 209.
    • Del Val C, Barea JM, Azcón-Aguilar C (1999) Diversity of arbuscular mycorrhizal fungus populations in heavy-metal-contaminated soils. Applied and Environmental Microbiology, 65, 718 - 723.
    • Edwards SG, Fitter AH, Young JPW (1997) Quantification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction. Mycological Research, 101, 1440 - 1444.
    • Eom A-H, Hartnett DC, Wilson GWT (2000) Host plant species effects on arbuscular mycorrhizal fungal communities in tallgrass prairie. Oecologia, 122, 435 - 444.
    • Gehrig H, Schüßler A, Kluge M (1996) Geosiphon pyriforme, a fungus forming endocytobiosis with Nostoc (cyanobacteria), is an ancestral member of the Glomales: evidence by SSU rRNA analysis. Journal of Molecular Evolution, 43, 71 - 81.
    • van der Heijden MGA, Boller T, Wiemken A, Sanders IR (1998a) Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology, 79, 2082 - 2091.
    • van der Heijden MGA, Klironomos JN, Ursic M et al. (1998b) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature, 396, 69 - 72.
    • Helgason T, Daniell TJ, Husband R, Fitter AH, Young JPW (1998) Ploughing up the wood-wide web? Nature, 394, 431.
    • Helgason T, Fitter AH, Young JPW (1999) Molecular diversity of arbuscular mycorrhizal fungi colonising Hyacinthoides nonscripta (bluebell) in a seminatural woodland. Molecular Ecology, 8, 659 - 666.
    • Hodge A, Campbell CD, Fitter AH (2001) An arbuscular mycorrhizal fungus accelerates decomposition and acquires nitrogen directly from organic material. Nature, 413, 297 - 299.
    • Johnson NC, Graham JH, Smith FA (1997) Functioning of mycorrhizal associations along the mutualism-parasitism continuum. New Phytologist, 135, 575 - 585.
    • Kenny C (2000) Field Data Handbook. The Macauley Land Use Research Institute NERC Soil Biodiversity Thematic Programme, Sourhope, Kelso, Scotland, UK.
    • Kuhn G, Hijri M, Sanders IR (2001) Evidence for the evolution of multiple genomes in arbuscular mycorrhizal fungi. Nature, 414, 745 - 748.
    • Losos JB (1996) Phylogenetic perspectives on community ecology. Ecology, 77, 1344 - 1354.
    • Lloyd-Macgilp SA, Chambers SM, Dodd JC et al. (1996) Diversity of the ribosomal internal transcribed spacers within and among isolates of Glomus mosseae and related mycorrhizal fungi. New Phytologist, 133, 103 - 111.
    • Maidak BL, Cole JR, Lilburn TG et al. (2001) The RDP-II (Ribosomal Database Project). Nucleic Acids Research, 29, 173 - 174.
    • Merryweather J, Fitter AH (1998) The arbuscular mycorrhizal fungi of Hyacinthoides non-scripta I. Diversity of fungal taxa. New Phytologist, 138, 117 - 129.
    • Mishler BD, Brandon RN (1987) Individuality, pluralism, and the phylogenetic species concept. Biology and Philosophy, 2, 397 - 414.
    • Mishler BD, Donoghue MJ (1982) Species concepts: a case for pluralism. Systematic Zoology, 31, 491 - 503.
    • Morton JB, Bentivenga SP, Bever JD (1995) Discovery, measurement, and interpretation of diversity in arbuscular endomycorrhizal fungi (Glomales, Zygomycetes). Canadian Journal of Botany, 73 (Suppl. 1), S25 - S32.
    • Newsham KK, Fitter AH, Watkinson AR (1995) Arbuscular mycorrhiza protect an annual grass from root pathogenic fungi in the field. Journal of Ecology, 83, 991 - 1000.
    • Redecker D, Morton JB, Bruns TD (2000) Ancestral lineages of arbuscular mycorrhizal fungi (Glomales). Molecular Phylogenetics and Evolution, 14, 276 - 284.
    • Saitou N, Nei M (1987) The Neighbor-Joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406 - 425.
    • Sanders IR, Alt M, Groppe K, Boller T, Wiemken A (1995) Identification of ribosomal DNA polymorphisms among and within spores of the Glomales - application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytologist, 130, 419 - 427.
    • Schluter D (2000) The ecology of adaptive radiation. In: Oxford Series in Ecology and Evolution (eds May RM, Harvey PH), Oxford University Press, Oxford.
    • Schüßler A, Gehrig H, Schwarzott D, Walker C (2001a) Analysis of partial Glomales SSU rRNA gene sequences: implications for primer design and phylogeny. Mycological Research, 105, 5 - 15.
    • Schüßler A, Schwarzott D, Walker C (2001b) A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycological Research, 105, 1413 - 1421.
    • Simon L, Bousquet J, Lévesque RC, Lalonde M (1993) Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature, 363, 67 - 69.
    • Simon L, Lalonde M, Bruns TD (1992) Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonising roots. Applied and Environmental Microbiology, 58, 291 - 295.
    • Smith SE, Read DJ (1997) Mycorrhizal Symbiosis, 2nd edn. Academic Press, San Diego.
    • Streitwolf-Engel R, Boller T, Wiemken A, Sanders IR (1997) Clonal growth traits of two Prunella species are determined by cooccurring arbuscular mycorrhizal fungi from a calcareous grassland. Journal of Ecology, 85, 181 - 191.
    • Swofford DL (1993) PAUP: Phylogenetic Analysis Using Parsimony, Version 3.1.1. Illinois Natural History Survey, Champaign.
    • Tehler A, Farris JS, Lipscomb DL, Källersjö M (2000) Phylogenetic analyses of the fungi based on large rDNA data sets. Mycologia, 93, 459 - 474.
    • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24, 4876 - 4882.
    • Torsvik V, Goksøyr J, Daae FL (1990) High diversity in DNA of soil bacteria. Applied and Environmental Microbiology, 56, 782 - 787.
    • Vandenkoornhuyse P, Leyval C (1998) SSU rDNA sequencing and PCR-fingerprinting reveal genetic variation within Glomus mosseae. Mycologia, 90, 791- 797.
    • Walker C, Trappe JM (1993) Names and epithets in the Glomales and Endogonales. Mycological Research, 97, 339 - 344.
    • Wilkinson DM (1997) The role of seed dispersal in the evolution of mycorrhizae. Oikos, 78, 394 - 396.
    • Young FW, Harris DF (1994) Multidimensional scaling. In: SPSS Professional Statistics 6.1 (ed. Norusis JJ), pp. 155 - 222. SPSS Inc., Chicago, IL, USA.
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