LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Sparks, A.M.; Betson, M.; Oviedo, G.; Sandoval, C.; Cooper, P.J.; Stothard, J.R. (2014)
Publisher: Cambridge University Press
Journal: Journal of Helminthology
Languages: English
Types: Article
Subjects: qx_200, qx_277, wc_870, wa_395, Short Communication

Classified by OpenAIRE into

mesheuropmc: parasitic diseases
To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with RsaI and HaeIII, PCR-RFLP analysis revealed the presence of A. lumbricoides in people and A. suum in pigs in Ecuador. In contrast, while there are no pigs on Zanzibar, of the 56 worms obtained from people, one was genotyped as A. suum. No additional genetic variation was detected upon further PCR-RFLP analysis with several other restriction enzymes. Upon measurement, worm mass and length differed by location and by species, A. suum being lighter and longer. While there is no evidence to suggest zoonotic transmission in Ecuador, an enduring historical signature of previous zoonotic transmission remains on Zanzibar.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • Anderson, T.J.C. (1995) Ascaris infections in humans from North America: molecular evidence for crossinfection. Parasitology 110, 215 - 219.
    • Anderson, T.J.C. (2001) The dangers of using single locus markers in parasite epidemiology: Ascaris as a case study. Trends in Parasitology 17, 183 - 188.
    • Arizono, N., Yoshimura, Y., Tohzaka, N., Yamada, M., Tegoshi, T., Onishi, K. & Uchikawa, R. (2010) Ascariasis in Japan: is pig-derived Ascaris infecting humans? Japanese Journal of Infectious Diseases 63, 447- 448.
    • Bendall, R.P., Barlow, M., Betson, M., Stothard, J.R. & Nejsum, P. (2011) Zoonotic ascariasis, United Kingdom. Emerging Infectious Diseases 17, 1964 - 1966.
    • Bethony, J., Brooker, S., Albonico, M., Geiger, S.M., Loukas, A., Diemert, D. & Hotez, P.J. (2006) Soiltransmitted helminth infections: ascariasis, trichuriasis, and hookworm. The Lancet 367, 1521 - 1532.
    • Betson, M., Halstead, F.D., Nejsum, P., Imison, E., Khamis, I.S., Sousa-Figueiredo, J.C., Rollinson, D. & Stothard, J.R. (2011) A molecular epidemiological investigation of Ascaris on Unguja, Zanzibar using isoenzyme analysis, DNA barcoding and microsatellite DNA profiling. Transactions of the Royal Society of Tropical Medicine & Hygiene 105, 370 - 379.
    • Betson, M., Nejsum, P. & Stothard, J.R. (2013) From the twig tips to the deeper branches: new insights into evolutionary history and phylogeography of Ascaris. pp. 266 - 287 in Holland, C. (Ed.) Ascaris: the neglected parasite. Oxford, Elsevier.
    • Betson, M., Nejsum, P., Bendall, R.P., Deb, R.M. & Stothard, J.R. (2014) Molecular epidemiology of ascariasis: A global perspective on the transmission dynamics of Ascaris in people and pigs. Journal of Infectious Diseases [Epub ahead of print].
    • Brooker, S.J. & Pullan, R.L. (2013) Ascaris lumbricoides and ascariasis: estimating number infected and burden of disease. pp. 343 - 362 in Holland, C. (Ed.) Ascaris: the neglected parasite. Oxford, Elsevier.
    • Cavallero, S., Snabel, V., Pacella, F., Perrone, V. & D'Amelio, S. (2013) Phylogeographical studies of Ascaris spp. based on ribosomal and mitochondrial DNA sequences. PLoS Neglected Tropical Diseases 7, e2170.
    • Cooper, P.J., Chico, M.E., Bland, M., Griffin, G.E. & Nutman, T.B. (2003) Allergic symptoms, atopy, and geohelminth infections in a rural area of Ecuador. American Journal of Respiratory and Critical Care Medicine 168, 313 - 317.
    • de Silva, N.R., Chan, M.S. & Bundy, D.A.P. (1997) Morbidity and mortality due to ascariasis: reestimation and sensitivity analysis of global numbers at risk. Tropical Medicine and International Health 2, 519 - 528.
    • Draper, N.R. & Smith, H. (1998) Applied regression analysis. 3rd edn. 706 pp. Chichester, Wiley.
    • Galvin, T.J. (1968) Development of human and pig Ascaris in the pig and rabbit. The Journal of Parasitology 54, 1085 - 1091.
    • In˜ iguez, A.M., Leles, D., Jaeger, L.H., Carvalho-Costa, F.A., Arau´ jo, A. & Amazonas Research Group (2012) Genetic characterisation and molecular epidemiology of Ascaris spp. from humans and pigs in Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 106, 604 - 612.
    • Leles, D., Gardner, S.L., Reinhard, K., In˜ iguez, A. & Araujo, A. (2012) Are Ascaris lumbricoides and Ascaris suum a single species? Parasite & Vectors 5, 42.
    • Liu, G.H., Wu, C.Y., Song, H.Q., Wei, S.J., Xu, M.J., Lin, R.Q., Zhao, G.H., Huang, S.Y. & Zhu, X.Q. (2012) Comparative analyses of the complete mitochondrial genomes of Ascaris lumbricoides and Ascaris suum from human and pigs. Gene 492, 110 - 116.
    • Moncayo, A.L., Vaca, M., Erazo, S., Oviedo, G., Rodriguez, A., Chico, M.E., Gomez, E., Barreto, M.L. & Cooper, P.J. (2008) Impact of long-term treatment with ivermectin on geohelminth infections in Esmeraldas Province, Ecuador. PLoS Neglected Tropical Diseases 2, e293.
    • Nejsum, P., Parker, E.D. Jr, Frydenberg, J., Roepstorff, A., Boes, J., Haque, R., Astrup, I., Prag, J. & Sørensen, S. (2005) Ascariasis is a zoonosis in Denmark. Journal of Clinical Microbiology 43, 1142 - 1148.
    • Stothard, J.R., Imison, E., French, M.D., Sousa-Figueiredo, J.C., Khamis, I.S. & Rollinson, D. (2008) Soiltransmitted helminthiasis among mothers and their pre-school children on Unguja Island, Zanzibar with emphasis upon ascariasis. Parasitology 135, 1447 - 1455.
    • Takata, I. (1951) Experimental infection of man with Ascaris of man and the pig. Kitasato Archives of Experimental Medicine 23, 151 - 159.
    • Zhou, C., Li, M., Yuan, K., Deng, S. & Peng, W. (2012) Pig Ascaris: an important source of human ascariasis in China. Infection, Genetics and Evolution 12, 1172 - 1177.
  • No related research data.
  • No similar publications.

Share - Bookmark

Funded by projects

  • WT | Impact of early infectious a...

Cite this article