LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Kowalska, Marta; Tian, Faming; Šmehilová, Mária; Galuszka, Petr; Frébort, Ivo; Napier, R. (Richard); Dale, Nicholas (2011)
Publisher: Elsevier Science BV
Languages: English
Types: Article
Subjects: QK

Classified by OpenAIRE into

mesheuropmc: food and beverages, fungi, heterocyclic compounds, macromolecular substances
An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • [1] O. Hedden, Ann. Rev. Plant Physiol. Plant Mol. Biol. 44 (1993) 107-130.
    • [2] G.C. Davis, M.B. Hein, B.C. Neely, C.R. Sharp, M.G. Carnes, Anal. Chem. 63 (1985) 639A648A.
    • [3] J. Olsson, K. Claeson, B. Karlberg, A.C. Nordström, J. Chromatogr. A 824 (1998) 231-239.
    • [4] B. Sotta, G. Pillate, F. Pelese, I. Sabbagh, M. Bonnet, R. Maldiney, Plant Physiol. 84 (1987) 571- 573.
    • [5] H. Wabiko, M. Minemura, Plant Physiol. 112 (1996) 939-951.
    • [6] L. River, A. Crozier, Principles and Practice of Hormone Analysis, Academic Press, London, 1987
    • [7] E.W. Weiler, J. Eberle, R. Mertens, R. Atzon, M. Feyerabend, P.S. Jordan, A. Arnscheidt, U. Wieczorek, Immunology in Plant Science, Cambridge Press, Cambridge, 1986.
    • [8] E.M.S. MacDonail, R.O. Morris, Methods Ezymol. 110 (1985) 347-358.
    • [9] L. Clark, C. Lyons, Ann. N. Y. Acad. Sci. 148 (1962) 133-153.
    • [10] M. Pohanka, P. Skládal, J. Applied Biomed. 6 (2008) 57-64.
    • [11] E. Bakker, Electrochemical sensors. Anal. Chem. 76 (2004) 3285-3298.
    • [12] A.A. Karyakin, E.E. Karyakin, L. Gorton, Anal. Chem. 72 (2000) 1720-1723.
    • [13] Q. Deng, S.J. Dong, J. Electroanal. Chem. 377 (1994) 191-195.
    • [14] L. Chen, W. Gorski, Anal. Chem. 73 (2001) 2862-2868.
    • [15] X. Wei, J. Cruz, W. Gorski, Anal. Chem. 74 (2002) 5039-5046.
    • [16] X. Wei, M. Zhang, W. Gorski, Anal. Chem. 75 (2003) 2060-2064.
    • [17] I. Gill, A. Ballesteros, Trends Biotechnol. 18 (2000) 282- 296.
    • [18] R. Gupta, N.K. Chaudhury, Biosens. Bioelectron. 22 (2007) 2387-2399.
    • [19] F. Tian, W. Wu, M. Broderick, V. Vamvakaki, N. Chaniotakis, N. Dale, Biosens. Bioelectron. 25 (2010) 2408-2013.
    • [20] D.W. Mok, M.C. Mok, Annu. Rev. Plant Physiol. Plant Mol. Biol. 52 (2001) 89-118.
    • [21] J. Li, L-T. Xiao, G-M. Zeng, G-H. Huang, G-L. Shen, R-Q. Yu, Anal. Biochem. 321 (2003) 89- 95.
    • [22] B.G. Brownlee, R.H. Hall, C.D. Whitty, Can. J. Biochem. 53 (1975) 37-41.
    • [23] H. Popelková, M.W. Fraaije, O. Novák, J. Frébortová, K.D. Bilyeu, I. Frébort, Biochem. J. 398 (2006) 113-124.
    • [24] J. Frébortová, O. Novák, I. Frébort, R. Jorda, Plant J. 61 (2010) 467-481.
    • [25] J. Frébortová, P. Galuszka, T. Werner, T. Schmülling, I. Frébort, Biol. Plant. 51 (2007) 673-682.
    • [26] T. Werner, V. Motyka, M. Strnad, T. Schmülling, Proc. Natl. Acad. Sci. U.S.A. 98 (2001) 10487- 10492.
    • [27] J.D. Bendtsen, H. Nielsen, G. von Heijne, S. Brunak, Mol. Biol. 340 (2004) 783-795.
    • [28] M. Kowalska, P. Galuszka, J. Frébortová, M. Šebela, T. Béres, T. Hluska, M. Šmehilová, K.D. Bilyeu, I Frébort, Phytochemistry (2010), in press
    • [29] M.M. Bradford, Anal. Biochem. 72 (1976) 248-254.
    • [30] J. Lin-Cereghino, J.M. Cregg, FEMS Microbiol. Rev. 24 (2000) 45-66.
    • [31] G.P. Lin-Cereghino, J. Lin-Cereghino, Ch. Ilgen, J.M. Cregg, Curr. Opinion Biotechnol. 13 (2002) 329-332.
    • [32] J. Lin-Cereghino, G.P. Lin-Cereghino, in: J.M. Cregg (Ed.), Pichia Protocols. Humana Press, Totowa, New Jersey, 2007, pp. 11-27.
    • [33] X. Wang, H. Gu, F. Yin, Y. Tu, Biosens. Bioelectron. 24 (2009) 1527-1530.
    • [34] F. Tian, A.V. Gourine, R.T.R. Huckstepp, N. Dale, Anal. Chim. Acta 645 (2009) 86-91.
    • [35] M. Klotz, A. Ayral, C. Guizard, L. Cot, Bull. Korean Chem. Soc. 20 (1999) 879-884.
    • [36] B. Yin, R. Yuan, Y. Chai, S. Chen, S. Cao, Y. Xu, P. Fu, Biotechnol Lett 30 (2008) 317-322.
    • [37] J. Kulys, R. Stupak, Open Nanosci. J. 2 (2008) 34-38.
    • [38] F. Ricci, G. Palleschi, Biosens. Bioelectron. 21 (2005) 389-407.
    • [39] F. Tian, E. Llaudet, N. Dale, Anal. Chem. 27 (2007) 6760-6766.
    • [40] P. Galuszka, I. Frébort, M. Šebela, P. Sauer, S. Jacobsen, P. Peč, Eur. J. Biochem. 268 (2001) 450-461.
    • [41] K.D. Bilyeu, J.L. Cole, J.G. Laskey, W.R. Riekhof, T.J. Esparza, M.D. Kramer, R.O. Morris, Plant Physiol. 125 (2001) 378-386.
    • [42] J.G. Laskey, P. Patterson, K. Bilyeu, R.O. Morris, Plant Growth Regul. 40 (2003) 189-196.
    • [43] J. Frébortová, M.W. Fraaije, P. Galuszka, M. Šebela, P. Peč, J. Hrbáč, K.D. Bilyeu, J.T. English, I. Frébort, Biochem. J. 380 (2004) 121-130.
    • [44] J.C. Goodrick, M. Xu, R. Finnegan, S Schilling, S. Schiavi, H. Hoppe, N.C. Wan, Biotechnol. Bioeng. 74 (2001) 492-497.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article