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Bradford, Claire M.; Ramos, Inês; Cross, Alison K.; Haddock, Gail; McQuaid, Stephen; Nicholas, Anthony P.; Woodroofe, M. Nicola (2014)
Publisher: Elsevier BV
Journal: Journal of Neuroimmunology
Languages: English
Types: Article
Subjects: Clinical Neurology, Immunology and Allergy, Immunology, Neurology
Multiple sclerosis (MS) is a chronic inflammatory neurodegenerative disease, considered to be autoimmune in origin. Post-translational modification of central nervous system proteins, including glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP), through citrullination of arginine residues, may lead to exposure of neoepitopes, triggering autoimmunity. Here we investigated the expression of citrullinated proteins in active MS lesions, MS normal appearing white matter and control brain white matter. We demonstrate increased citrullinated GFAP and MBP by immunohistochemistry and western blotting in areas of ongoing demyelination, suggesting a pivotal role for deimination of GFAP and MBP in MS pathogenesis MS.
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