Remember Me
Or use your Academic/Social account:


Or use your Academic/Social account:


You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.


Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message


Verify Password:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Thorpe, Abbey; Creasey, Stuart; Sammon, Chris; Le Maitre, Christine (2016)
Publisher: A O Foundation
Languages: English
Types: Article
Bone loss associated with degenerative disease and trauma\ud is a clinical problem increasing with the aging population.\ud Thus, effective bone augmentation strategies are required;\ud however, many have the disadvantages that they require\ud invasive surgery and often the addition of expensive\ud growth factors to induce osteoblast differentiation. Here,\ud we investigated a Laponite crosslinked, pNIPAMDMAc\ud copolymer (L-pNIPAM-co-DMAc) hydrogel\ud with hydroxyapatite nanoparticles (HAPna), which can\ud be maintained as a liquid ex vivo, injected via narrowgauge\ud needle into affected bone, followed by in situ\ud gelation to deliver and induce osteogenic differentiation\ud of human mesenchymal stem cells (hMSC). L-pNIPAMco-DMAc\ud hydrogels were synthesised and HAPna added\ud post polymerisation. Commercial hMSCs from one donor\ud (Lonza) were incorporated in liquid hydrogel, the mixture\ud solidified and cultured for up to 6 weeks. Viability of hMSCs\ud was maintained within hydrogel constructs containing\ud 0.5 mg/mL HAPna. SEM analysis demonstrated matrix\ud deposition in cellular hydrogels which were absent in\ud acellular controls. A significant increase in storage modulus\ud (G’) was observed in cellular hydrogels with 0.5 mg/mL\ud HAPna. Semi-quantitative immunohistochemistry and\ud histological analysis demonstrated that bone differentiation\ud markers and collagen deposition was induced within 48 h,\ud with increased calcium deposition with time. The thermally\ud triggered hydrogel system, described here, was sufficient\ud without the need of additional growth factors or osteogenic\ud media to induce osteogenic differentiation of commercial\ud hMSCs. Preliminary data presented here will be expanded\ud on multiple patient samples to ensure differentiation is seen\ud in these samples. This system could potentially reduce\ud treatment costs and simplify the trea
  • The results below are discovered through our pilot algorithms. Let us know how we are doing!

    • Nakano T, Kaibara K, Tabata Y, Nagata N, Enomoto S, Sen M, Miclau T (2007) Autologous iliac crest bone Marukawa E, Umakoshi Y (2002) Unique alignment angdraft: should it still be the gold standard for treating texture of biological apatite crystallites in typicalncoanlucniioinse?d. Injury 38: S75-S80. tissues analyzed by microbeam X-ray diffractometer Shapiro F (2008) Bone development and its relation to system. Bone 31: 479-487. fracture repair. The role of mesenchymal osteoblasts and Nassr A, Lee JY, Bashir RS, Rihn JA, Eck JC, Kang JD, surface osteoblasts. Eur Cell Mater 15: 53-76. Lim MR (2009) Does incorrect level needle localization Shin H, Temenoff JS, Mikos AG (200In3)vitro during anterior cervical discectomy and fusion lead to cytotoxicity of unsaturated oligo [poly (ethylene glycol) accelerated disc degeneration? Spine (Phila Pa 1976) 34: fumarate] macromers and their cross-linked hydrogels. 189-192. Biomacromolecules 4: 552-560.
    • Nilsson KG, Kärrholm J, Carlsson L, Dalén T (1999) Sinha A, Das G, Kumar Sharma B, Prabahan Roy R, Hydroxyapatite coatinvgersus cemented ixation of the Kumar Pramanick A, Nayar S (2007) Poly (vinyl alcohol)- tibial component in total knee arthroplasty: prospective hydroxyapatite biomimetic scafold for tissue regeneration. randomized comparison of hydroxyapatite-coated and Materials Science and Engineering: C 27: 70-74. cemented tibial components with 5-year follow-up using Sodek J, Cheifetz S (2000) Molecular regulation of radiostereometry. J Arthroplast1y4: 9-20. osteogenesis. Bone engineering 1: 31-43.
    • Owen SC, Shoichet MS (2010) Design of three- Stein GS, Lian JB, Van Wijnen AJ, Stein JL, Montecino dimensional biomimetic scafolds. J Biomed MaterMR,esJAaved A, Zaidi SK, Young DW, Choi J, Pockwinse 94: 1321-1331. SM (2004) Runx2 control of organization, assembly and Pan Y, Xiong D, Gao F (2008) Viscoelastic behaviaocrtivity of the regulatory machinery for skeletal gene of nano-hydroxyapatite reinforced poly (vinyl alcohol) gel expression. Oncogen2e3: 4315-4329. biocomposites as an articular cartilage. J Mater Sci MaterStolzing A, Jones E, McGonagle D, Scutt A (2008) Med 19: 1963-1969. Age-related changes in human bone marrow-derived Park H, Temenof JS, Tabata Y, Caplan AI, Mikos AG mesenchymal stem cells: consequences for cell therapies. (2007) Injectable biodegradable hydrogel composites for Mech Ageing Dev 129: 163-173. rabbit marrow mesenchymal stem cell and growth factor Sun JS, Tsuang YH, Liao CJ, Liu HC, Hang YS, Lin FH delivery for cartilage tissue engineering. Biomaterials 28: (1997) The efects of calcium phosphate particles on the 3217-3227. growth of osteoblasts. J Biomed Mater R3es7: 324-334.
    • Pourati J, Maniotis A, Spiegel D, Schafer JL, ButlerThorpe AA, Boyes V, Sammon C, Le Maitre C (2016) JP, Fredberg JJ, Ingber DE, Stamenovic D, Wang N Thermally triggered injectable hydrogel, which induces (1998) Is cytoskeletal tension a major determinant of cell mesenchymal stem cell diferentiation to nucleus pulposus deformability in adherent endothelial cells?. Am J Pcheylslis: oPlotential for regeneration of the intervertebral disc. 274: C1283-C1289. Acta Biomater 36: 99-111.
    • Qing F, Wang Z, Hong Y, Liu M, Guo B, Luo H, Zhang Trappmann B, Gautrot JE, Connelly JT, Strange DG, Li X (2012) Selective efects of hydroxyapatite nanoparticlYe,sOyen ML, Cohen Stuart MA, Boehm H, Li B, Vogel V, on osteosarcoma cells and osteoblasts. J Mater Sci MaterSpatz JP, Watt FM, Huck WT (2012) Extracellular-matrix Med 23: 2245-2251. tethering regulates stem-cell fate. Nat Mater 11: 642-649.
    • Rea SM, Best SM, Bonield W (2004) Bioactivity of Venugopal J, Prabhakaran MP, Zhang Y, Low S, Choon ceramic-polymer composites with varied composition and AT, Ramakrishna S (2010) Biomimetic hydroxyapatitesurface topography. J Mater Sci Mater Med15: 997-1005. containing composite nanoibrous substrates for bone Reilly DT, BursteinAH, Frankel VH (1974) The elastiticssue engineering. Philos Trans A Math Phys Eng Sci 368: modulus for bone. J Biomec7h: 271-275. 2065-2081.
    • Rho JY, Ashman RB, Turner CH (1993) Young's Wang JH, Jia F, Gilbert TW, Woo SL (2003) Cell modulus of trabecular and cortical bone material: ultrasonic orientation determines the alignment of cell-produced and microtensile measurements. J Biom2e6ch: 111-119. collagenous matrix. J Biom3e6c:h97-102.
    • Rho JY, Kuhn-Spearing L, Zioupos P (1998) Wang LS, Du C, Chung JE, Kurisawa M (2012) Mechanical properties and the hierarchical structure of Enzymatically cross-linked gelatin-phenol hydrogels with bone. Med Eng Phys 20: 92-102. a broader stifness range for osteogenic diferentiation of Rho J, Tsui TY, Pharr GM (1997) Elastic properties ohfuman mesenchymal stem cells. Acta Biomater 8: 1826- human cortical and trabecular lamellar bone measured by 1837. nanoindentation. Biomaterials 18: 1325-1330. Wei J, He P, Liu A, Chen X, Wang X, Jing X (2009) Sakaguchi Y, Sekiya I, Yagishita K, Muneta T (200S5)urface Modiication of Hydroxyapatite Nanoparticles Comparison of human stem cells derived from various with Thermal-Responsive PNIPAM by ATRP. Macromol mesenchymal tissues: superiority of synovium as a cell Biosci 9:1237-1246. source. Arthritis Rheum 52: 2521-2529. Weiner S, Traub W (1992) Bone structure: from Saunders JM, Tong T, Le Maitre CL, Freemont TJ, angstroms to microns. FASEB J6: 879-885. Saunders BR (2007) A study of pH-responsive microgel Willenbacher N (1996) Unusual thixotropic properties dispersions: from luid-to-gel transitions to mechaonfiacqauleous dispersions of laponite RD. J Colloid Interface property restoration for load-bearing tissue. Soft Matter Sci 182: 501-510. 3: 486-494. Xavier JR, Thakur T, Desai P, Jaiswal MK, Sears N, Cosgrif-Hernandez E, Kaunas R, Gaharwar AK (2015)
  • No related research data.
  • No similar publications.

Share - Bookmark

Funded by projects

Cite this article