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Airley, R; Evans, AR; Mobasheri, A; Hewitt, SM
Languages: English
Types: Article
Subjects: RC0254
The hypoxic tumor microenvironment is associated with malignant progression and poor treatment response. The glucose transporter Glut-1 is a prognostic factor and putative hypoxia marker. So far, studies of Glut-1 in cancer have utilised conventional immunohistochemical analysis in a series of individual biopsy or surgical specimens. Tissue microarrays, however, provide a rapid, inexpensive means of profiling biomarker expression. To evaluate hypoxia markers, tissue cores must show architectural features of hypoxia, i.e. viable tissue surrounding necrotic regions. Glut-1 may be a useful biomarker to validate tissue microarrays for use in studies of hypoxia-regulated genes in cancer. In this study, we carried out immunohistochemical detection of Glut-1 protein in many tumor and normal tissue types in a range of tissue microarrays. Glut-1 was frequently found in peri-necrotic regions, occurring in 9/34 lymphomas, 6/12 melanomas, and 5/16 glioblastomas; and in 43/54 lung, 22/84 colon, and 23/60 ovarian tumors. Expression was rare in breast (6/40) and prostate (1/57) tumors, and in normal tissue, was restricted to spleen, tongue and CNS endothelium. In conclusion, tissue microarrays enable the observation of Glut-1 expression in peri-necrotic regions, which may be linked to hypoxia, and reflect previous studies showing differential Glut-1 expression across tumor types and non malignant tissue.
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    • Ahmed, N., Berridge, M.V., 1997. Regulation of glucose transport by interleukin-3 in growth factordependent and oncogene-transformed bone marrow-derived cell lines. Leuk Res. 21, 609-618.
    • Airley, R., Loncaster, J., Davidson, S., Bromley, M., Roberts, S., Patterson, A., Hunter, R., Stratford, I., West, C., 2001. Glucose transporter glut-1 expression correlates with tumor hypoxia and predicts metastasis-free survival in advanced carcinoma of the cervix. Clin Cancer Res. 7, 928-934.
    • Airley, R.E., Loncaster, J., Raleigh, J.A., Harris, A.L., Davidson, S.E., Hunter, R.D., West, C.M., Stratford, I.J., 2003. GLUT-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: relationship to pimonidazole binding. Int J Cancer. 104, 85-91.
    • Airley, R.E., Phillips, R.M., Evans, A.E., Double, J., Burger, A.M., Feibig, H.H., West, C.M., Stratford, I.J., 2005. Hypoxia-regulated glucose transporter Glut-1 may influence chemosensitivity to some alkylating agents: results of EORTC (First Translational Award) study of the relevance of tumour hypoxia to the outcome of chemotherapy in human tumour-derived xenografts. Int J Oncol. 26, 1477-1484.
    • Albertella, M.R., Loadman, P.M., Jones, P.H., Phillips, R.M., Rampling, R., Burnet, N., Alcock, C., Anthoney, A., Vjaters, E., Dunk, C.R., Harris, P.A., Wong, A., Lalani, A.S., Twelves, C.J., 2008. Hypoxia-selective targeting by the bioreductive prodrug AQ4N in patients with solid tumors: results of a phase I study. Clin Cancer Res. 14, 1096-1104.
    • Amann, T., Maegdefrau, U., Hartmann, A., Agaimy, A., Marienhagen, J., Weiss, T.S., Stoeltzing, O., Warnecke, C., Scholmerich, J., Oefner, P.J., Kreutz, M., Bosserhoff, A.K., Hellerbrand, C., 2009. GLUT1 expression is increased in hepatocellular carcinoma and promotes tumorigenesis. Am J Pathol. 174, 1544-1552.
    • Baron-Delage, S., Mahraoui, L., Cadoret, A., Veissiere, D., Taillemite, J.L., Chastre, E., Gespach, C., Zweibaum, A., Capeau, J., Brot-Laroche, E., Cherqui, G., 1996. Deregulation of hexose transporter expression in Caco-2 cells by ras and polyoma middle T oncogenes. Am J Physiol. 270, G314-323.
    • Behrooz, A., Ismail-Beigi, F., 1997. J Biol Chem. 272, 5555-5562.
    • Binder, C., Binder, L., Marx, D., Schauer, A., Hiddemann, W., 1997. Deregulated simultaneous expression of multiple glucose transporter isoforms in malignant cells and tissues. Anticancer Res. 17, 4299- 4304.
    • Brizel, D.M., Scully, S.P., Harrelson, J.M., Layfield, L.J., Bean, J.M., Prosnitz, L.R., Dewhirst, M.W., 1996. Tumor oxygenation predicts for the likelihood of distant metastases in human soft tissue sarcoma. Cancer Res. 56, 941-943.
    • Brown, R.S., Wahl, R.L., 1993. Overexpression of Glut-1 glucose transporter in human breast cancer. An immunohistochemical study. Cancer. 72, 2979-2985.
    • Cantuaria, G., Magalhaes, A., Penalver, M., Angioli, R., Braunschweiger, P., Gomez-Marin, O., Kanhoush, R., Gomez-Fernandez, C., Nadji, M., 2000. Expression of GLUT-1 glucose transporter in borderline and malignant epithelial tumors of the ovary. Gynecol Oncol. 79, 33-37.
    • Chen, C., Pore, N., Behrooz, A., Ismail-Beigi, F., Maity, A., 2001. Regulation of glut1 mRNA by hypoxiainducible factor-1. Interaction between H-ras and hypoxia. J Biol Chem. 276, 9519-9525.
    • Chung, F.Y., Huang, M.Y., Yeh, C.S., Chang, H.J., Cheng, T.L., Yen, L.C., Wang, J.Y., Lin, S.R., 2009. GLUT1 gene is a potential hypoxic marker in colorectal cancer patients. BMC Cancer. 9, 241.
    • Cooper, R., Sarioglu, S., Sokmen, S., Fuzun, M., Kupelioglu, A., Valentine, H., Gorken, I.B., Airley, R., West, C., 2003. Glucose transporter-1 (GLUT-1): a potential marker of prognosis in rectal carcinoma? Br J Cancer. 89, 870-876.
    • Denekamp, J., Dasu, A., 1999. Inducible repair and the two forms of tumour hypoxia--time for a paradigm shift. Acta Oncol. 38, 903-918.
    • Fogt, F., Wellmann, A., Urbanski, S.J., Noffsinger, A., Poremba, C., Zimmerman, R.L., Alsaigh, N., 2001. Glut-1 expression in dysplastic and regenerative lesions of the colon. Int J Mol Med. 7, 615-619.
    • Furudoi, A., Tanaka, S., Haruma, K., Yoshihara, M., Sumii, K., Kajiyama, G., Shimamoto, F., 2001. Clinical significance of human erythrocyte glucose transporter 1 expression at the deepest invasive site of advanced colorectal carcinoma. Oncology. 60, 162-169.
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