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Dehn, Sabrina; Castelletto, Valeria; Hamley, Ian W.; Perrier, Sébastien (2012)
Publisher: American Chemical Society
Languages: English
Types: Article
Subjects:
Identifiers:doi:10.1021/bm3007117
A strategy is presented that exploits the ability of\ud synthetic polymers of different nature to disturb the strong selfassembly\ud capabilities of amyloid based β-sheet forming peptides.\ud Following a convergent approach, the peptides of interest were\ud synthesized via solid-phase peptide synthesis (SPPS) and the\ud polymers via reversible addition−fragmentation chain transfer\ud (RAFT) polymerization, followed by a copper(I) catalyzed azide−\ud alkyne cycloaddition (CuAAC) to generate the desired peptide−\ud polymer conjugates. This study focuses on a modified version of\ud the core sequence of the β-amyloid peptide (Aβ), Aβ(16−20) (KLVFF). The influence of attaching short poly(Nisopropylacrylamide)\ud and poly(hydroxyethylacrylate) to the peptide sequences on the self-assembly properties of the hybrid\ud materials were studied via infrared spectroscopy, TEM, circular dichroism and SAXS. The findings indicate that attaching these\ud polymers disturbs the strong self-assembly properties of the biomolecules to a certain degree and permits to influence the\ud aggregation of the peptides based on their β-sheets forming abilities. This study presents an innovative route toward targeted and\ud controlled assembly of amyloid-like fibers to drive the formation of polymeric nanomaterials.
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    • (1) Klok, H. A. J. Polym. Sci. A 2005, 43, 1-17.
    • (2) Drotleff, S.; Lungwitz, U.; Breunig, M.; Dennis, A.; Blunk, T.; Tessmar, J.; Göpferich, A. European Journal of Pharmaceutics and Biopharmaceutics 2004, 58, 385-407.
    • (3) Klok, H.-A. Macromolecules 2009, 42, 7990-8000.
    • (4) Rösler, A.; Klok, H. A.; Hamley, I. W.; Castelletto, V.; Mykhaylyk, O. O.
    • Biomacromolecules 2003, 4, 859-863.
    • (5) Hamley, I. W. Angew. Chem. Int. Ed. 2007, 46, 8128-8147.
    • (6) Hartgerink, J. D.; Beniash, E.; Stupp, S. I. Science 2001, 294, 1684-1688.
    • (7) Reches, M.; Gazit, E. Science 2003, 300, 625-627.
    • (8) Yang, Y. L.; Khoe, U.; Wang, X. M.; Horii, A.; Yokoi, H.; Zhang, S. G. Nano Today 2009, 4, 193-210.
    • (9) Ulijn, R. V.; Smith, A. M. Chem. Soc. Rev. 2007, 37, 664-675.
    • (10) Zelzer, M.; Ulijn, R. V. Chem. Soc. Rev. 2010, 39, 3351-3357.
    • (11) Gauthier, M. A.; Klok, H. A. Chem. Comm. 2008, 2591-2611.
    • (12) Aggeli, A.; Bell, M.; Boden, N.; Keen, J. N.; Knowles, P. F.; McLeish, T. C. B.; Pitkeathly, M.; Radford, S. E. Nature 1997, 386, 259-262.
    • (13) Dobson, C. M. Nature 2003, 426, 884.
    • (14) Goedert, M.; Spillantini, M. G. Science 2006, 314, 777.
    • (15) McLean, P. J.; Kawamata, H.; Hyman, B. T. Neuroscience 2001, 104, 901- 912.
    • (16) Kahn, S. E.; Andrikopoulos, S.; Verchere, C. B. Diabetes 1999, 48, 241-253.
    • (17) Chimon, S.; Shaibat, M. A.; Jones, C. R.; Calero, D. C.; Aizezi, B.; Ishii, Y.
    • Nature Structural and Molecular Biology 2007, 14, (12), 1157-1164.
    • (18) Tjernberg, L. O.; Lilliehook, C.; Callaway, D. J. E.; Naslund, J.; Hahne, S.; J., T.; Terenius, L.; Nordtsedt, C. J. Biol. Chem. 1997, 272, 12601.
    • (19) Krysmann, M. J.; Castelletto, V.; Hamley, I. W. Soft Matter 2007, 3, 1401- 1406.
    • (20) Krysmann, M. J.; Castelletto, V.; Kelarakis, A.; Hamley, I. W.; Hule, R. A.; Pochan, D. J. Biochemistry 2008, 47, 4597-4605.
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