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Masiga, D.K.; Ndung’u, K.; Tweedie, A.; Tait, A.; Turner, C. (2006)
Publisher: Elsevier
Languages: English
Types: Article
Subjects: QR355

Classified by OpenAIRE into

mesheuropmc: parasitic diseases, food and beverages, bacterial infections and mycoses, geographic locations
We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar (‘type A’) whereas 2 isolates differed substantially (‘type B’). Type A isolates have been generally regarded as genetically identical but AFLP analysis was able to identify multiple differences between them and split the type A T. evansi isolates into two distinct clades.
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