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Nagel, O.W.; Waldron, S.; Jones, H.G. (2001)
Publisher: American Society of Plant Biologists
Languages: English
Types: Article
Subjects: QK
In situ measurements of alternative respiratory pathway activity are needed to provide insight into the energy efficiency of plant metabolism under various conditions in the field. The only reliable method at present to measure alternative oxidase (AOX) activity is through measurement of changes in delta O-18(O-2), which to date has only been used in laboratory environments. We have developed a cuvette system to measure partitioning of electrons to AOX that is suitable for off-line use and for field experiments. Plant samples are enclosed in airtight cuvettes and O-2 consumption is monitored. Gas samples from the cuvette are stored in evacuated gas containers until measurement of delta O-18(O-2). We have validated this method using differing plant material to assess AOX activity. Fractionation factors were calculated from delta O-18(O-2) measurements, which could be measured with an accuracy and precision to 0.1 parts per thousand and 0.3 parts per thousand respectively. Potential sources of error are discussed and quantified. Our method provides results similar to those obtained with laboratory incubations on-line to a mass spectrometer but greatly increases the potential for adoption of the stable isotope method.
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    • Angert A, Luz B (2001) Fractionation of oxygen isotopes by root respiration: implications for the isotopic composition of atmospheric O2. Geochim Cosmochim Acta 65: 1695-1701
    • Atkin OK, Cummins WR, Collier DE (1993) Light induction of alternative pathway capacity in leaf slices of Belgium endive. Plant Cell Environ 16: 231-235
    • Azco´ n-Bieto J, Lambers H, Day DA (1983) Effect of photosynthesis and carbohydrate status on respiratory rates and the involvement of the alternative pathway in leaf respiration. Plant Physiol 72: 598-603
    • Bottinga Y, Craig H (1969) Oxygen isotope fractionation between CO2 and water, and the isotopic composition of marine atmospheric CO2. Earth Planet Sci Lett 5: 285-295
    • Collier DE, Cummins WR (1992) Stimulation of respiration during plant water deficits in sand-grown Arnica alpina. In H Lambers, LHW Van der Plas, eds, Biochemical and Physiological Aspects of Plant Respiration. SPB Academic Publishing bv, The Hague, The Netherlands, pp 541-546
    • Drake BG, Azco´ n-Bieto J, Berry J, Bunce J, Dijkstra P, Farrar J, Gifford RM, Gonza`lez-Meler MA, Koch G, Lambers H et al. (1999) Does elevated atmospheric CO2 concentration inhibit mitochondrial respiration in green plants? Plant Cell Environ 22: 649-657
    • Gale J, Reuveni J, Mayer AM (1992) An oxygen gradient method for determining the partitioning of respiration between its cytochrome and alternative pathway components, with application to Lemna gibba fronds under different temperatures. In H Lambers, LHW Van der Plas, eds, Biochemical and Physiological Aspects of Plant Respiration. SPB Academic Publishing bv, The Hague, The Netherlands, pp 433-442
    • Gonza`lez-Meler MA, Giles L, Thomas RB, Siedow JN (2001) Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply. Plant Cell Environ 24: 205-215
    • Gonza`lez-Meler MA, Ribas-Carbo M, Giles L, Siedow JN (1999) The effect of growth and measurement temperature on the activity of the alternative respiratory pathway. Plant Physiol 120: 765-772
    • Gonza`lez-Meler MA, Ribas-Carbo M, Siedow JN, Drake BG (1996) Direct inhibition of plant mitochondrial respiration by elevated CO2. Plant Physiol 112: 1349-1355
    • Guy RD, Berry JA, Fogel ML, Hoering TC (1989) Differential fractionation of oxygen isotopes by cyanideresistant and cyanide-sensitive respiration in plants. Planta 177: 483-491
    • Henry BK, Atkin OK, Day DA, Millar AH, Menz RI, Farquhar GD (1999) Calculation of the oxygen isotope discrimination factor for studying plant respiration. Aust J Plant Physiol 26: 773-780
    • Lennon AM, Neuenschwander UH, Ribas-Carbo M, Giles L, Ryals JA, Siedow JN (1997) The effects of salicylic acid and tobacco mosaic virus infection on the alternative oxidase of tobacco. Plant Physiol 115: 783-791
    • Millar AH, Atkin OK, Lambers H, Wiskich JT, Day DA (1995) A critique of the use of inhibitors to estimate partitioning of electrons between mitochondrial respiratory pathways in plants. Physiol Plant 95: 523-532
    • Millenaar FF, Roelofs R, Gonza`lez-Meler MA, Siedow JN, Wagner AM, Lambers H (2000) The alternative oxidase in roots of Poa annua after transfer from high-light to low-light conditions. Plant J 23: 623-632
    • Møller IM, Berczi A, Van der Plas LHW, Lambers H (1988) Measurement of the activity and capacity of the alternative pathway in intact plant-tissues: identification of problems and possible solutions. Physiol Plant 72: 642-649
    • Noguchi K, Go CS, Terashima I, Ueda S, Yoshinari T (2001) Activities of the cyanide-resistant respiratory pathway in leaves of sun and shade species. Aust J Plant Physiol 28: 27-35
    • Parsons R (1989) Physiological regulation of nitrogen fixation in soybean root nodules. PhD thesis. Australian National University, Canberra
    • Parsons HL, Yip JYH, Vanlerberge GC (1999) Increased respiratory restriction during phosphate-limited growth in transgenic tobacco cells lacking alternative oxidase. Plant Physiol 121: 1309-1320
    • Popp M, Lied W, Meyer J, Richter A, Schiller P, Schwitte H (1996) Sample preservation for determination of organic compounds: microwave versus freeze-drying. J Exp Bot 47: 1469-1474
    • Purvis AC, Shewfelt RL (1993) Does the alternative pathway ameliorate chilling injury in sensitive plant-tissues? Physiol Plant 88: 712-718
    • Ribas-Carbo M, Aroca R, Gonza`lez-Meler MA, Irigoyen JJ, Sanchez-Diaz M (2000a) The electron partitioning between the cytochrome and alternative respiratory pathways during chilling recovery in two cultivars of maize differing in chilling sensitivity. Plant Physiol 122: 199-204
    • Ribas-Carbo M, Berry JA, Azcon-Bieto J, Siedow JN (1994) The reaction of the plant mitochondrial cyanideresistant alternative oxidase with oxygen. Biochim Biophys Acta 1188: 205-212
    • Ribas-Carbo M, Berry JA, Yakir D, Giles L, Robinson SA, Lennon AM, Siedow JN (1995) Electron partitioning between the cytochrome and alternative pathways in plant-mitochondria. Plant Physiol 109: 829-837
    • Ribas-Carbo M, Lennon AM, Robinson SA, Giles L, Berry JA, Siedow JN (1997) The regulation of electron partitioning between the cytochrome and alternative pathways in soybean cotyledon and root mitochondria. Plant Physiol 113: 903-911
    • Ribas-Carbo M, Robinson SA, Gonza`lez-Meler MA, Lennon AM, Giles L, Siedow JN, Berry JA (2000b) Effects of light on respiration and oxygen isotope fractionation in soybean cotyledons. Plant Cell Environ 23: 983-989
    • Robinson SA, Ribas-Carbo M, Yakir D, Giles L, Reuveni Y, Berry JA (1995) Beyond SHAM and cyanide: opportunities for studying the alternative oxidase in plant respiration using oxygen-isotope discrimination. Aust J Plant Physiol 22: 487-496
    • Robinson SA, Yakir D, Ribas-Carbo M, Giles L, Osmond CB, Siedow JN, Berry JA (1992) Measurements of the engagement of cyanide-resistant respiration in the crassulacean acid metabolism plant Kalanchoe-daigremontiana with the use of online oxygen isotope discrimination. Plant Physiol 100: 1087-1091
    • Rychter AM, Mikulska M (1990) The relationship between phosphate status and cyanide-resistant respiration in bean roots. Physiol Plant 79: 663-667
    • Simons BH, Millenaar FF, Mulder L, Van Loon LC, Lambers H (1999) Enhanced expression and activation of the alternative oxidase during infection of Arabidopsis with Pseudomonas syringae pv tomato. Plant Physiol 120: 529-538
    • Sokal RR, Rohlf FJ (1995) Biometry: The Principles and Practice of Statistics in Biological Research, Ed 3. W.H. Freeman, New York
    • Stewart CR, Martin BA, Reding L, Cerwick S (1990) Seedling growth, mitochondrial characteristics, and alternative respiratory capacity of corn genotypes differing in cold tolerance. Plant Physiol 92: 761-766
    • Theodorou ME, Plaxton WC (1993) Metabolic adaptations of plant respiration to nutritional phosphate deprivation. Plant Physiol 101: 339-344
    • Vanlerberghe GC, McIntosh L (1992) Lower growth temperature increases alternative pathway capacity and alternative oxidase protein in tobacco. Plant Physiol 100: 115-119
    • Wilson SB (1988) The switching of electron flux from the cyanide-insensitive oxidase to the cytochrome pathway in mung-bean (Phaseolus aureus L) mitochondria. Biochem J 249: 301-303
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