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Carr, Stephen B.; Mecia, Lauren B.; Phillips, Simon E. V.; Thomas, Christopher D. (2013)
Publisher: International Union of Crystallography
Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Languages: English
Types: Article
Subjects: plasmid pSTK1, plasmid pT181, replication initiation proteins, Crystallization Communications, Geobacillus stearothermophilus, rolling-circle replication, Staphylococcus aureus
Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 family, which replicate via a rolling-circle mechanism mediated by a plasmid-encoded replication initiation protein. Currently, there is no structural information available for the pT181-family Rep proteins. Here, the crystallization of a catalytically active fragment of a homologous replication initiation protein from the thermophile Geobacillus stearothermophilus responsible for the replication of plasmid pSTK1 is reported. Crystals of the RepSTK1 fragment diffracted to a resolution of 2.5 Å and belonged to space group P212121.
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