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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Yun Mai; Guangxia Gao
Publisher: Public Library of Science (PLoS)
Journal: PLoS ONE
Languages: English
Types: Article
Subjects: Human Genetics, Research Article, Biology, Viruslike Particles, Infectious Diseases, Microbiology, Gene Therapy, Medicine, Viral Vectors, Viral Diseases, Viral Replication, Q, R, Genetics, HIV, Virology, Vector Biology, Science, Viral Packaging, Viral Nucleic Acid

Classified by OpenAIRE into

mesheuropmc: viruses
Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding protein 1 (IMP1) enhanced the production of infectious MLV vector. Overexpression of IMP1 increased the stability of viral genomic RNA in virus producer cells and packaging of the RNA into progeny virus in a dose-dependent manner. Downregulation of IMP1 in virus producer cells resulted in reduced production of the retroviral vector. These results indicate that IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors.

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