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Baruffi,Marcelo Razera; Engel,Edgard Edward; Squire,Jeremy Andrew; Tone,Luis Gonzaga; Rogatto,Silvia Regina (2003)
Publisher: Sociedade Brasileira de Genética
Languages: English
Types: Article
Subjects: QH426-470, Genetics, CGH, Ewing's Sarcoma, comparative genomic hybridization, osteosarcoma
We applied a combination of comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH), to characterize the genetic aberrations in three osteosarcomas (OS) and one Ewing's sarcoma. CGH identified recurrent chromosomal losses at 10p14-pter and gains at 8q22.3-24.1 in OS. Interphase FISH allowed to confirm 8q gain in two cases. A high amplification level of 11q12-qter was detected in one OS. The Ewing's sarcoma showed gain at 1p32-36.1 as the sole chromosome alteration. These studies demonstrate the value of molecular cytogenetic methods in the characterization of recurrent genomic alterations in bone tumor tissue.
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