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Hu, Hanyang; Lu, Xing; Cen, Xiang; Chen, Xiaohua; Li, Feng; Zhong, Shan (2014)
Publisher: Hindawi Publishing Corporation
Journal: BioMed Research International
Languages: English
Types: Article
Subjects: R, Research Article, Medicine, Article Subject
Cadmium is a common toxicant that is detrimental to many tissues. Although a number of transcriptional signatures have been revealed in different tissues after cadmium treatment, the genes involved in the cadmium caused male reproductive toxicity, and the underlying molecular mechanism remains unclear. Here we observed that the mice treated with different amount of cadmium in their rodent chow for six months exhibited reduced serum testosterone. We then performed RNA-seq to comprehensively investigate the mice testicular transcriptome to further elucidate the mechanism. Our results showed that hundreds of genes expression altered significantly in response to cadmium treatment. In particular, we found several transcriptional signatures closely related to the biological processes of regulation of hormone, gamete generation, and sexual reproduction, respectively. The expression of several testosterone synthetic key enzyme genes, such as Star, Cyp11a1, and Cyp17a1, were inhibited by the cadmium exposure. For better understanding of the cadmium-mediated transcriptional regulatory mechanism of the genes, we computationally analyzed the transcription factors binding sites and the mircoRNAs targets of the differentially expressed genes. Our findings suggest that the reproductive toxicity by cadmium exposure is implicated in multiple layers of deregulation of several biological processes and transcriptional regulation in mice.
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    • Satarug, S, Moore, MR. Adverse health effects of chronic exposure to low-level cadmium in foodstuffs and cigarette smoke. Environmental Health Perspectives . 2004; 112 (10): 1099-1103
    • Fowler, BA. Monitoring of human populations for early markers of cadmium toxicity: a review. Toxicology and Applied Pharmacology . 2009; 238 (3): 294-300
    • Waisberg, M, Joseph, P, Hale, B, Beyersmann, D. Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology . 2003; 192 (2-3): 95-117
    • Jin, YH, Clark, AB, Slebos, RJC. Cadmium is a mutagen that acts by inhibiting mismatch repair. Nature Genetics . 2003; 34 (3): 326-329
    • Wang, B, Li, Y, Tan, Y. Low-dose Cd induces hepatic gene hypermethylation, along with the persistent reduction of cell death and increase of cell proliferation in rats and mice. PLoS ONE . 2012; 7 (3)
    • Akinloye, O, Arowojolu, AO, Shittu, OB, Anetor, JI. Cadmium toxicity: a possible cause of male infertility in Nigeria. Reproductive Biology . 2006; 6 (1): 17-30
    • Thompson, J, Bannigan, J. Cadmium: toxic effects on the reproductive system and the embryo. Reproductive Toxicology . 2008; 25 (3): 304-315
    • Ronco, AM, Montenegro, M, Castillo, P. Maternal exposure to cadmium during gestation perturbs the vascular system of the adult rat offspring. Toxicology and Applied Pharmacology . 2011; 251 (2): 137-145
    • Hanson, ML, Holásková, I, Elliott, M, Brundage, KM, Schafer, R, Barnett, JB. Prenatal cadmium exposure alters postnatal immune cell development and function. Toxicology and Applied Pharmacology . 2012; 261 (2): 196-203
    • Dakeshita, S, Kawai, T, Uemura, H. Gene expression signatures in peripheral blood cells from Japanese women exposed to environmental cadmium. Toxicology . 2009; 257 (1-2): 25-32
    • Li, G-Y, Kim, M, Kim, J-H, Lee, M-O, Chung, J-H, Lee, B-H. Gene expression profiling in human lung fibroblast following cadmium exposure. Food and Chemical Toxicology . 2008; 46 (3): 1131-1137
    • Trapnell, C, Roberts, A, Goff, L. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nature Protocols . 2012; 7 (3): 562-578
    • Chen, LY, Wei, K-C, Huang, AC-Y. RNASEQR-a streamlined and accurate RNA-seq sequence analysis program. Nucleic Acids Research . 2012; 40 (6, article e42)
    • Eisen, MB, Spellman, PT, Brown, PO, Botstein, D. Cluster analysis and display of genome-wide expression patterns. Proceedings of the National Academy of Sciences of the United States of America . 1998; 95 (25): 14863-14868
    • Huang, DW, Sherman, BT, Lempicki, RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature Protocols . 2009; 4 (1): 44-57
    • Zhang, B, Kirov, S, Snoddy, J. WebGestalt: an integrated system for exploring gene sets in various biological contexts. Nucleic Acids Research . 2005; 33 (2): W741-W748
    • Shamir, R, Maron-Katz, A, Tanay, A. EXPANDER—an integrative program suite for microarray data analysis. BMC Bioinformatics . 2005; 6, article 232
    • Cline, MS, Smoot, M, Cerami, E. Integration of biological networks and gene expression data using Cytoscape. Nature Protocols . 2007; 2 (10): 2366-2382
    • Livak, KJ, Schmittgen, TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods . 2001; 25 (4): 402-408
    • Obholz, KL, Akopyan, A, Waymire, KG, MacGregor, GR. FNDC3A is required for adhesion between spermatids and Sertoli cells. Developmental Biology . 2006; 298 (2): 498-513
    • Yang, F, Eckardt, S, Leu, NA, McLaughlin, KJ, Wang, PJ. Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis. Journal of Cell Biology . 2008; 180 (4): 673-679
    • Reijo, R, Lee, T-Y, Salo, P. Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nature Genetics . 1995; 10 (4): 383-393
    • Ruggiu, M, Speed, R, Taggart, M. The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis. Nature . 1997; 389 (6646): 73-77
    • Lin, Y, Page, DC. Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL/6 mice. Developmental Biology . 2005; 288 (2): 309-316
    • Deshpande, S, Agosti, V, Manova, K, Moore, MAS, Hardy, MP, Besmer, P. Kit ligand cytoplasmic domain is essential for basolateral sorting in vivo and has roles in spermatogenesis and hematopoiesis. Developmental Biology . 2010; 337 (2): 199-210
    • Luoh, S-W, Bain, PA, Polakiewicz, RD. Zfx mutation results in small animal size and reduced germ cell number in male and female mice. Development . 1997; 124 (11): 2275-2284
    • Heemers, HV, Tindall, DJ. Androgen receptor (AR) coregulators: a diversity of functions converging on and regulating the AR transcriptional complex. Endocrine Reviews . 2007; 28 (7): 778-808
    • Ji, Y-L, Wang, H, Liu, P. Pubertal cadmium exposure impairs testicular development and spermatogenesis via disrupting testicular testosterone synthesis in adult mice. Reproductive Toxicology . 2010; 29 (2): 176-183
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