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Engel , Karl-Heinz; Demmel , Anja; Ehlert , Alexandra; Hupfer , Christine; Busch , Ulrich (2009)
Publisher: HAL CCSD
Languages: English
Types: Article
Subjects: Life Sciences

Classified by OpenAIRE into

mesheuropmc: food and beverages, digestive, oral, and skin physiology
International audience; The simultaneous detection of DNA from different potentially allergenic food ingredients by a ligation-dependent probe amplification (LPA) system is described. The approach allows the detection of several targets in a one-tube assay. Synthetic oligonucleotides were designed to detect DNA from peanut, cashew nut, pecan nut, pistachio nut, hazelnut, sesame seeds, macadamia nut, almond, walnut and brazil nut. The specificity of the system was tested with DNA from more than 50 plant and animal species. The sensitivity of the method was shown to be suitable to detect allergenic ingredients in the lower ppm range. The limit of detection (LOD) for the detection of single allergens in different food matrices was determined to 5 mg/kg. The novel analytical strategy represents a useful tool for the surveillance of the established legislation on food allergens in the European Union.
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    • 2000/13/EC as regards certain food ingredients. Official Journal of the O
    • European Communities 28.11.2007, L 310, pp 11-14. n
    • Ehlert A, Hupfer C, Demmel A, Engel KH, Busch U. 2008a. Detection of cashew l
    • 2002. Relative quantification of 40 nucleic acid sequences by multiplex ligation-
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