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Nivia Graciele V. Pinto; Carla L. S. Mota; Regina Cely R. Barroso; Silvana Moreira; Delson Braz
Publisher: Elsevier
Journal: Revista Brasileira de Hematologia e Hemoterapia
Languages: English
Types: Article
Subjects: fluorescência, fluorescence, Diseases of the blood and blood-forming organs, Blood cells, RC633-647.5, radiação ionizante, radiation, ionizing, Células sanguíneas
A técnica de fluorescência de raios X por reflexão total usando radiação síncrotron (SR-TXRF) é uma poderosa ferramenta utilizada para a determinação das concentrações elementares presentes em amostras biológicas. O objetivo deste estudo é avaliar as possíveis alterações causadas por processos de irradiação na concentração de elementos-traço em amostras de sangue humano. As amostras de sangue foram coletadas no Laboratório de Análises Clínicas Dr. Elilel Figueiredo, Rio de Janeiro, e divididas em dois grupos. O primeiro grupo foi irradiado com doses de 1.500, 2.500 e 3.000 cGy, utilizando o irradiador Gammacell 220 Excel, e o segundo foi irradiado com doses que variaram de 2 cGy a 100 cGy, utilizando uma bomba de cobalto Theratron 780 C do Inca, Rio de Janeiro. Todas as amostras de sangue total, plasma e matriz celular foram então liofilizadas e, em seguida, passaram pelo procedimento padrão de digestão. Todas as medidas foram realizadas na linha de fluorescência de raios X do Laboratório Nacional de Luz Síncrotron (LNLS), em Campinas, Brasil. Não se verificou variação significativa na concentração de Ca e, em contrapartida, o K foi o único elemento que sofreu alterações significativas para todas as amostras analisadas em função da dose. A concentração de Fe diminuiu apenas para as amostras de sangue total e plasma. A concentração de Zn apresentou uma diminuição significativa somente para as amostras de sangue total.
Total-reflection X-ray fluorescence using synchrotron radiation (SR-TXRF) is a powerful analytical technique to study trace elements in biomedical samples. The aim of this study was to investigate possible changes in essential trace element concentrations caused by irradiation procedures. Fresh blood samples were obtained from the Dr. Eliel Figueiredo Laboratory, Rio de Janeiro. The samples were separated in two groups. The first was irradiated with doses of 1500, 2500 and 3000cGy, using a Gammacell 220 Exce irradiator, and the second was irradiated with doses of from 2 to 100 cGy, using gamma radiation in a Theraton 780 C irradiator. After irradiation, all samples were lyophilized to remove the water and then submitted to standard chemical digestion by adding nitric acid. All the measurements were carried out by X-Ray Fluorescence beamline at the Brazilian Synchrotron Light Laboratory (LNLS) in Campinas. While no positive association was found for variations in calcium, the variations in potassium were positively associated with the radiation dose for all analyzed samples. On the other hand, iron levels decreased for whole blood and plasma and zinc levels decreased only in whole blood as the dose increased.
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