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Azam Mehrbakhsh; Monir Doudi; Hossein Motamedi (2016)
Publisher: BioIT InternationalsJournals
Journal: International Journal of Advanced Biotechnology and Research
Languages: English
Types: Article
Subjects: Separation, Identification, Bacteria, Resistance, Pb, 16S rRNA., Biotechnology, TP248.13-248.65, Chemical technology, TP1-1185, Technology, T
Heavy metals are one of the pollution sources in environment. The pollution due to these metals is the problem that could have negative impact on water. Human is faced with these poisons effects due to occupational reasons. The lead is regarded as heavy metal whose industrial applications cause environmental pollution in high rate.The aim of this project was Separation and Molecular Identification of Resistant Bacteria to Lead from Behbahan Bidboland Gas Refinery Wastewater (Iran). For this aim, 6 samples of water and refinery output wastewater is provided after sampling, pH, COD, BOD rates and lead metal primary concentration was determined in wastewaters, after this phase the concentration and separation of resistant bacteria was done to separate them from lead. In this phase the minimum inhibitory concentration was defined from heavy metal growth (MIC). To determine the bacteria identity first the evaluation method of bacteria morphologic and gram staining and then biochemical tests and finally colony-–PCR molecular method was used and then separated bacteria 16S rRNA gen was identified. The obtained results from this research showed that among the separated bacteria from Behbahan Bidboland gas refinery wastewater, the Providencia genous had the most resistant toward Pb. The MIC rate for this bacteria was calculated at 1140 mg/l. the result of this study showed that separated bacteria in this wastewater seemed to have high resistant toward heavy metals due to long encountering to pollution sources and hence they could be proper factor for bioremediation process of heavy metals
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