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Soliman, Zeinab; Moubasher, A. H.; Abdel-Sater, M. A. (2017)
Publisher: JBBooks
Types: Book
Subjects: fungi, yeast, Citrus, grapevine
ABSTRACT 3 HISTORICAL REVIEW 5 1. Air-borne yeast fungi 9 2. Yeast fungi recovered from soils 10 3. Phyllosphere and phylloplane yeast fungi 12 4. Carposphere and carpoplane yeast fungi 13 5. Yeast fungi recovered from juice 15 OBJECTIVES OF THIS STUDY 18 METHODOLOGY 19 1. Sampling location 19 2. Collection of samples 19 3. Isolation of air-borne yeast fungi 23 4. Isolation of soil yeast fungi 24 A. Determination of soil moisture content (MC) 24 B. Determination of soil pH 24 C. Isolation of soil yeast fungi 24 5. Isolation of phyllosphere yeast fungi 25 6. Isolation of phylloplane yeast fungi 25 7. Isolation of carposphere yeast fungi 26 8. Isolation of carpoplane yeast fungi 26 9. Isolation of juice yeast fungi 26 10. Media used for isolation of yeast fungi from different sources 27 A. Dichloran yeast extract malt extract agar (DYM) 27 B. Dichloran Rose Bengal chloramphenicol agar (DRBC) (King et al. 1979) 27 11. Identification of yeasts 27 A. Morphological characters 27 B. Physiological characters 28 COMPARISON BETWEEN YEAST BIOTA RECOVERED FROM DIFFERENT SOURCES OF CITRUS AND GRAPEVINE PLANTATIONS 37 1. List of identified yeast species 37 2. Air of citrus and grapevine 39 3. Soil of citrus and grapevine plantations 40 4. Phyllosphere of citrus and grapevine 47 5. Phylloplane of citrus and grapevine 51 6. Carposphere of citrus and grape fruits 54 7. Carpoplane of citrus and grape fruits 59 8. Juice of citrus and grape fruits 62 9. Yeast fungi associated with different growth stages of leaf and fruit 65 A. In grape phyllosphere 65 B. In grape phylloplane 66 C. In citrus carposphere 67 D. In citrus carpoplane 67 E. In grape carposphere 68 F. In grape carpoplane 69 DIVERSITY, BIOCHEMICAL AND MOLECULAR CHARACTERISATION OF YEAST SPECIES RECORDED FROM CITRUS AND GRAPEVINE PLANTATIONS 71 1. Ascomyceteous yeasts 71 Ambrosiozyma J.P. van der Walt 71 Aureobasidium Viala & Boyer (as Aureobasidium sp.) 71 Candida Berkhout 71 Debaryomyces Lodder & Kreger-van Rij 73 Geotrichum Link 75 Hanseniaspora Berkh. 76 Issatchenkia Kudriavzev 81 Klyuveromyces Van der Walt 82 Kodemaea Y. Yamada, Tom. Suzuki, M. Matsuda & Mikata 83 Pichia E. C. Hansen 84 Physiological tests 87 Genotypic identification of ascomyceteous yeasts 90 2. Basidiomyceteous yeasts 104 Cryptococcus Vuillemin 104 Filobasidium L. S. Olive 113 Melanopsichium Beck 113 Pseudozyma Bandoni 113 Rhodotorula F. C. Harrison 115 Rhodosporidium Banno 120 Sporidiobolus Nyland 121 Sporobolomyces roseus Kluyver & van Niel 122 Trichosporon Behrend 123 Black yeasts 132 SUMMARY 144 REFERENCES 150
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