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Araújo, Danielle Silva (2014)
Publisher: Universidade Federal de Goiás
Languages: Portuguese
Types: Master thesis
Subjects: Parede celular, Proteoma, Micélio, Levedura, Cell wall, Proteome, Mycelium, Yeast, CIENCIAS BIOLOGICAS
Paracoccidioidomicose é uma importante micose sistêmica na América latina, com alta incidência no Brasil, Argentina, Colômbia e Venezuela. A doença é atribuída ao fungo termodimórfico Paracoccidioides spp.. Durante o processo infecioso podemos destacar o papel desempenhado pela parede celular, estrutura esta, vital para o crescimento, sobrevivência, e morfogênese do fungo, a qual está em constante mudança em resposta a sinais do ambiente. O interesse no estudo da parede celular de fungos ocorre principalmente pela ausência dessa estrutura nas células de mamíferos; por esse motivo os componentes da parede são alvos promissores para o desenvolvimento de novas drogas. Para descrever o perfil das proteínas constituintes da parede celular (PPCs) de Paracoccidioides sp. nas formas leveduriforme e miceliana, foi utilizada uma abordagem proteômica combinando cromatografia líquida em nanoescala com espectrometria de massas multiplexada (nanoUPLC-MSE). Entre as proteínas identificadas foi encontrada uma transglicosidase, homologa a Crh1p, correspondendo a uma proteína GPI ancorada, conhecida por estar envolvida em ligar quitina à β-glucana. Adesinas anteriormente descritas em Paracoccidioides sp. como enolase, gliceraldeído-3- fosfato desidrogenase, álcool desidrogenase, frutose-1,6-bifosfato aldolase e algumas chaperonas também foram identificadas. Além disso, nós identificamos a formamidase que tem sido descrita como localizada na parede celular e pode estar envolvida no metabolismo de nitrogênio, além de contribuir com propriedades antigênicas. Paracoccidioidomycosis (PCM) is the important systemic mycosis in Latin America, with high incidence in Brazil, Argentina, Colombia and Venezuela. The disease has been attributed to the thermodimorphic fungus Paracoccidioides sp.. During the infective process, we can highlight the role of the cell wall, which is a dynamic structure, vital for growth, survival and morphogenesis of the fungus. In addition, this structure is constantly changing in response to environmental signals and different stages of the cycle of the fungus. The interest in the fungal cell wall occurs primarily by lack of this structure in mammalian cells; for this reason cell wall components are promising targets for antifungal drug design. In order to describe the profile of cell wall proteins (CWPs) of Paracoccidioides sp., it was used a proteomic approach coupling nanoscale liquid chromatography to multiplexed mass spectrometry (nanoUPLC-MSE) to identify the CWPs isolated from Paracoccidioides sp. yeast cells and mycelia. Among the identified proteins, it was found a transglycosylase orthologue to the Crh1p, which is a GPI anchored protein, known to be involved in attaching chitin to β-glucan. Adhesins previously described in Paracoccidioides sp. such as enolase, glyceraldehyde-3-phosphate, alcohol dehydrogenase, fructose-1,6-biphosphate aldose and some chaperones were also identified. Moreover, we identified formamidase that was previously described as localized in the fungus cell wall and may be involved in nitrogen metabolism besides contributing with antigenic properties.
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