LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Ribeiro, Luiz Fernando; Bedendo, Ivan Paulo (2006)
Publisher: Sociedade Brasileira de Fitopatologia
Languages: Portuguese
Types: Unknown
Subjects: molicutes, amarelos, Euphorbia pulcherrima, Mollicutes, yellows
Bico-de-papagaio é uma espécie ornamental, encontrada tanto na forma de arbustos, presente em jardins residenciais e públicos, como comercializada em vasos para decoração de interiores. Com o objetivo de detectar a presença de fitoplasma em plantas mostrando sintomas de proliferação de ramos, amostras foram obtidas de plantas sintomáticas e assintomáticas cultivadas em jardins e de plantas comerciais sintomáticas envasadas. A técnica de duplo PCR, utilizando os iniciadores R16mF2/mR1 e R16F2n/R2, foi empregada para detecção do fitoplasma, a partir de DNA extraído de folhas. Fitoplasma foi detectado consistentemente em todas as amostras sintomáticas, porém não houve detecção em qualquer das amostras assintomáticas. A identificação molecular conduzida através de PCR com iniciadores específicos e por análise de RFLP revelou que o fitoplasma detectado pertence ao grupo 16SrIII. Assim, foi demonstrado que a ocorrência de proliferação de ramos em bico-de-papagaio está associada à presença de fitoplasma. Plantas superbrotadas apresentam um dossel mais compacto, sendo mais atrativas ao consumidor, portanto a presença do fitoplasma é considerada como benéfica para fins ornamentais e comerciais. Poinsettia is an ornamental species found as a shrub in residential and public gardens or commercialized in pots for indoor decoration. In order to detect phytoplasma in poinsettia exhibiting shoot proliferation, samples were collected from plants with and without symptoms grown in gardens, and commercial plants cultivated in pots showing symptoms. The PCR technique using primers R16mF2/mR1 and R16F2n/R2 was employed to detect phytoplasma by using DNA extracted from fresh leaves. The results revealed that phytoplasma was consistently detected from samples with symptoms, but no phytoplasma was found in any samples without symptoms. It was thus demonstrated that poinsettia proliferation was associated with the presence of phytoplasma. Molecular identification using group-specific primer pairs and RFLP analyses revealed that the phytoplasma was a member of group 16SrIII. Plants with proliferation have shown a compact top and are more attractive to consumers; hence the presence of phytoplasma is desirable for ornamental and commercial purposes.
  • No references.
  • No related research data.
  • No similar publications.

Share - Bookmark

Cite this article