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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Cho, Ho-Seong; Park, Suk-Jun; Park, Nam-Yong (2006)
Publisher: Canadian Veterinary Medical Association
Languages: English
Types: Article
Subjects: Short Communications
Identifiers:pmc:PMC1477935
A 1-step reverse-transcription polymerase chain reaction (RT-PCR) assay using TaqMan minor-groove-binding (MGB) probes was developed to distinguish between vaccine-type and wild-type strains of Classical swine fever virus (CSFV) in Korea. Because attenuated Korean LOM strains have been used in animal vaccination in Korea for some time but CSF remains a serious problem, there was a need for a practical approach to differentiating vaccine and field strains. We examined the fluorescence of 5 vaccine strains, 10 field strains, and 5 mixed samples. Three clusters of the samples could be distinguished: those with only fluorescence of the vaccine-type-specific probe, VIC; those with only fluorescence of the wild-type-specific probe, FAM; and those with both VIC and FAM fluorescence. The RT-PCR assay with fluorogenic probes is sensitive and accurate and is therefore useful for differentiating vaccine and field strains of CSFV in Korea.
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