LOGIN TO YOUR ACCOUNT

Username
Password
Remember Me
Or use your Academic/Social account:

CREATE AN ACCOUNT

Or use your Academic/Social account:

Congratulations!

You have just completed your registration at OpenAire.

Before you can login to the site, you will need to activate your account. An e-mail will be sent to you with the proper instructions.

Important!

Please note that this site is currently undergoing Beta testing.
Any new content you create is not guaranteed to be present to the final version of the site upon release.

Thank you for your patience,
OpenAire Dev Team.

Close This Message

CREATE AN ACCOUNT

Name:
Username:
Password:
Verify Password:
E-mail:
Verify E-mail:
*All Fields Are Required.
Please Verify You Are Human:
fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Poursani, Ensieh M.; Mehravar, Majid; Shahryari, Alireza; Mowla, Seyed Javad; Mohammad Soltani, Bahram (2017)
Publisher: Avicenna Research Institute
Journal: Avicenna Journal of Medical Biotechnology
Languages: English
Types: Article
Subjects: Short Communication, Alternative splicing, Genes, 5′ untranslated regions
Identifiers:pmc:PMC5650738
Background: The human OCT4 gene, responsible for pluripotency and self-renewal of Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells, can generate several transcripts (OCT4A, OCT4B-variant 2, OCT4B-variant 3, OCT4B-variant 5, OCT4B1, OCT4 B2 and OCT4B3) by alternative splicing and alternative promoters. OCT4A that is responsible for ES and EC cell stemness properties is transcribed from a promoter upstream of Exon1a in those cells. The OCT4B group variants (OCT4B-variant2, OCT4B-variant3, OCT4B-variant5, OCT4B1, OCT4B2 and OCT4B3) are transcribed from a different promoter located in intron 1 and some of them respond to the cell stresses, but cannot sustain the ES/EC cell self-renewal. However, the exact function of OCT4B group variants is still unclear. Methods: In the present study, we employed RT-PCR and sequencing approaches to explore different forms of OCT4 transcripts. Results: Our data revealed that the OCT4B group variants (OCT4B-variant2, OCT4 B-variant3, OCT4B1, OCT4B2 and OCT4B3) have longer 5′ UTR in the human bladder carcinoma cell line of 5637. Conclusion: These OCT4 variants undergo alternative splicing in their 5′ UTR which might exert regulatory roles in transcription and translation mechanisms.
  • No references.
  • No related research data.
  • No similar publications.

Share - Bookmark

Download from

Cite this article

Collected from