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fbtwitterlinkedinvimeoflicker grey 14rssslideshare1
Xenopoulos, Panagiotis; Piggot, Patrick J. (2011)
Publisher: American Society for Microbiology
Languages: English
Types: Article
Subjects: Microbial Cell Biology

Classified by OpenAIRE into

mesheuropmc: fungi
Identifiers:pmc:PMC3133180
During spore formation, Bacillus subtilis divides asymmetrically, resulting in two cells with different fates. Immediately after division, the transcription factor σF becomes active in the smaller prespore, followed by activation of σE in the larger mother cell. We recently showed that a delay in σE activation resulted in the novel phenotype of two spores (twins) forming within the same mother cell. Mother cells bearing twins are substantially longer than mother cells with single spores. Here we explore the regulation of the growth and DNA replication of the mother cell. We find that length correlates with chromosome number in the mother cell. We show that replication and growth could occur after asymmetric division in mother cells with no active σE. In contrast, when σE was active, replication and growth ceased. In growing mother cells, with no active σE, Spo0A-directed transcription levels remained low. In the presence of active σE, Spo0A-directed gene expression was enhanced in the mother cells. Artificial Spo0A activation blocked mother cell growth in the absence of σE. Spo0A activation blocked growth even in the absence of SirA, the Spo0A-directed inhibitor of the initiation of replication. Together, the results indicate that the burst of Spo0A-directed expression along with the activation of σE provides mechanisms to block the DNA replication and growth of the mother cell.
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